APO-BRDU FLOW CYTOMETRY KIT[APOPTOSIS TUNEL ASSAY FLUORESCENCE KIT]

• Catalog number
  MBS239287
• Price
  Please ask
• Size
  NA

• Products_type
  Kits
• Products_gene_name
  [APO-BRDU]
• Description
  This 1 is suited for programmed cell-death studies. FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors.
• Test
  MyBioSource supplies other types of Assays as 1. Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.

• Gene target
  APO-BRDU   KIT[   TUNEL   KIT]  
• Gene symbol
  KIT, TNFSF10, FAS, AOPEP, TNFRSF25
• Short name
  APO-BRDU KIT[APOPTOSIS TUNEL ASSAY KIT]
• Technique
  Flow Cytometry, apoptosis, Assay, Flow, apoptosis pathway, assays
• Host
  Assay
• Alternative name
  APO-Bromodeoxyuridine FLOW CYTOMETRY reagent[programmed cellular destruction TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling test FLUORESCENCE reagent]
• Alternative technique
  celldeath, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:6342
• Gene
  KIT
• Long gene name
  KIT proto-oncogene, receptor tyrosine kinase
• Synonyms gene
  • PBT
• Synonyms gene name
  • piebald trait
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Synonyms
  • CD117
  • SCFR
  • C-Kit
• Synonyms name
  • mast/stem cell growth factor receptor Kit
• GenBank acession
  • S67773
• Locus
  4q12
• Discovery year
  1986-01-01
• Entrez gene record
  3815
• Pubmed identfication
  • 9027509
• RefSeq identity
  • NM_000222
• Classification
  • Receptor tyrosine kinases
  • CD molecules
  • Immunoglobulin like domain containing
• VEGA ID
  OTTHUMG00000128713
• Locus Specific Databases
  • LRG_307

Gene info

• Identity
  HGNC:11925
• Gene
  TNFSF10
• Long gene name
  TNF superfamily member 10
• Synonyms gene name
  • tumor necrosis factor (ligand) superfamily, member 10
• Synonyms
  • TRAIL
  • Apo-2L
  • TL2
  • CD253
• GenBank acession
  • U37518
• Locus
  3q26
• Discovery year
  1998-12-04
• Pubmed identfication
  • 8777713
  • 8663110
• Classification
  • CD molecules
  • Tumor necrosis factor superfamily
• VEGA ID
  OTTHUMG00000156917

Gene info

• Identity
  HGNC:11920
• Gene
  FAS
• Long gene name
  Fas cell surface death receptor
• Synonyms gene
  • FAS1
  • APT1
  • TNFRSF6
• Synonyms gene name
  • tumor necrosis factor receptor superfamily, member 6
  • Fas (TNF receptor superfamily, member 6)
• Synonyms
  • CD95
  • APO-1
• Synonyms name
  • TNF receptor superfamily member 6
• GenBank acession
  • M67454
• Locus
  10q23.31
• Discovery year
  1992-06-25
• Entrez gene record
  355
• Pubmed identfication
  • 1385299
  • 1385309
• Classification
  • Tumor necrosis factor receptor superfamily
  • Death inducing signaling complex
  • CD molecules
• VEGA ID
  OTTHUMG00000018701
• Locus Specific Databases
  • Autoimmune Lymphoproliferative Syndrome Database (ALPSbase): Database of mutations causing human ALPS
  • LOVD at NCBI
  • LRG_134

Gene info

• Identity
  HGNC:1361
• Gene
  AOPEP
• Long gene name
  aminopeptidase O (putative)
• Synonyms gene
  • C9orf3
• Synonyms gene name
  • chromosome 9 open reading frame 3
• Synonyms
  • C90RF3
  • FLJ14675
  • APO
  • AP-O
• Synonyms name
  • aminopeptidase O
• GenBank acession
  • AF043896
• Locus
  9q22.32
• Discovery year
  1999-01-28
• Entrez gene record
  84909
• Pubmed identfication
  • 15687497
• RefSeq identity
  • NM_032823
• Classification
  • M1 metallopeptidases
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000020276

Gene info

• Identity
  HGNC:11910
• Gene
  TNFRSF25
• Long gene name
  TNF receptor superfamily member 25
• Synonyms gene
  • TNFRSF12
• Synonyms gene name
  • tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein)
  • tumor necrosis factor receptor superfamily, member 25
• Synonyms
  • DR3
  • TRAMP
  • WSL-1
  • LARD
  • WSL-LR
  • DDR3
  • TR3
  • APO-3
• GenBank acession
  • U72763
• Locus
  1p36.31
• Discovery year
  1998-12-04
• Entrez gene record
  8718
• Pubmed identfication
  • 9052839
  • 8934525
• RefSeq identity
  • NM_148965
• Classification
  • Tumor necrosis factor receptor superfamily
• VEGA ID
  OTTHUMG00000000831

MeSH Data

• Name
  Flow Cytometry
• Concept
  Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
• Tree numbers
  • E01.370.225.500.363.342
  • E01.370.225.500.386.350
  • E05.196.712.516.600.240.350
  • E05.200.500.363.342
  • E05.200.500.386.350
  • E05.242.363.342
  • E05.242.386.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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