anti- Phospho-CHEK1-S280 antibody

• Catalog number
  FNab09816
• Price
  Please ask
• Size
  2X100μg

• Form
  liquid
• Purification
  Immunogen affinity purified
• Purity
  ≥95% as determined by SDS-PAGE
• Storage
  PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
• Background
  The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
• Immunogen
  Phospho-CHEK1-S280
• Synonyms
  CHK1
• Calculated MW
  60 kDa
• Uniprot ID
  O14757
• Specificity
  Human, Mouse
• Tested Application
  ELISA, WB, IHC, IF
• Recommended dilution
  WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
• Image1
  Immunofluorescence analysis of MCF-7 cells using FNab09816. Blue: DAPI for nuclear staining.
• Image4
  HT-29 cells were subjected to SDS PAGE followed by western blot with FNab09816 at dilution of 1:1000
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Phospho-CHEK1-S280  
• Gene symbol
  CHEK1
• Short name
  anti- Phospho-CHEK1-S280 antibody
• Technique
  Antibody, anti, antibody to, antibodies against human proteins, antibodies for
• Host
  Rabbit
• Isotype
  IgG
• Alternative name
  antibody to- phosphorilated-CHEK1-S280 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:1925
• Gene
  CHEK1
• Long gene name
  checkpoint kinase 1
• Synonyms gene name
  • CHK1 (checkpoint, S.pombe) homolog
  • CHK1 checkpoint homolog (S. pombe)
• Synonyms
  • CHK1
• GenBank acession
  • AF016582
  • BC017575
• Locus
  11q24.2
• Discovery year
  1998-04-21
• Entrez gene record
  1111
• Pubmed identfication
  • 9278511
  • 9382850
• RefSeq identity
  • NM_001274
• VEGA ID
  OTTHUMG00000165853

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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