Anti-AFP Antibody (C3)
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Catalog numberA1423-100
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PricePlease ask
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Size100 µg
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Antibody TargetAlpha Fetoprotein
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Target Alternative NameAlpha fetoglobulin, FETA, HPAFP
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Antibody TypeMonoclonal
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Species ReactivitiesHuman, Monkey, Rat
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Immunogen SequenceAlpha fetoprotein (AFP) purified from serum of a hepatoma patient
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AccessionP02771
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Gene Id174
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AppearanceColorless liquid
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FormLiquid
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FormulationIn 10 mM PBS with 0.05% BSA & 0.05% azide
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PurificationProtein A/G purification
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ApplicationFC, IF, IHC
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Positive ControlIHC:Fetal liver tissue
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Application And UsagesFC: 0.5-1 ug/1X106 cells in 0.1 ml, IF: 0.5-1 ug/ml, IHC: 1-2 ug/ml
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DescriptionThis antibody recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
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Storage Condition-20°C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedNot Verified
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Immunocytochemistry VerifiedVerified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Dot Blot VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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Additional descriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolAFP
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Short nameAnti-AFP Antibody (C3)
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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IsotypeMouse IgG2b, kappa
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Alternative nameAntibody toAFP (antibody to-) (complement component 3)
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Alternative techniqueantibodies
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Alternative to gene targetcomplement component 3, AHUS5 and ARMD9 and ASP and C3a and C3b and CPAMD1 and HEL-S-62p, C3 and IDBG-21798 and ENSG00000125730 and 718, C5L2 anaphylatoxin chemotactic receptor binding, Extracellular, C3 and IDBG-193788 and ENSMUSG00000024164 and 102642251,12266, BT.19562 and IDBG-643930 and ENSBTAG00000017280 and 280677
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Gene info
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Identity
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Gene
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Long gene namealpha fetoprotein
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-04-01
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Entrez gene record
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Pubmed identfication
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data