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Category
Primary Antibodies
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Long description
CD4 (Cluster of Differentiation 4) is a glycoprotein expressed on the surface of T helper cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene. CD4 plays a role in the recognition of foreign antigens presented to T cells by MHC class II molecules. Furthermore, this antigen acts as a receptor for HIV-1 by binding the viral protein gp120. The CD4 antigen is present on most thymocytes and a subpopulation of peripheral blood T cells, called T helper cells (Th). In addition, CD4 is expressed on monocytes and weak on macrophages.
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Antibody come from
Edu-2 is a mouse monoclonal IgG2a antibody raised against CD4.
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Other description
Each vial contains 1 ml of 100µg/ml purified monoclonal antibody in 0.01 M sodium phosphate, 0.15 M NaCl, 0.2% BSA, 0.09% sodium azide; pH 7.3.
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Clone
Edu-2
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Antigen antibody binding interaction
Mouse Anti Human CD4 Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
CD4 (Edu-2) recognizes the CD4 antigen (a 55 kD glycoprotein).
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Research interest
CD Markers
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Application
Flow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin)
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Antibody s suited for
Flow cytometry and immunohistochemistry using frozen and paraffin embedded tissue sections. Optimal antibody dilution should be determined by titration. CD4 (Edu-2) is used in routine blood testing for CD4+ cells and CD4/CD8 ratios (e.g. HIV/AIDS patients) or as part of panels for the detection and differentiation of certain T cell leukemias. All these reagents are effectively formulated for indirect immunofluorescent staining of human blood for flow cytometric analysis using 10 µl/106 leukocytes. Staining with clone Edu-2 (CD4) monoclonal antibodies by flow cytometry analysis of normal blood cells is illustrated. Indirect staining was performed using 10 µl of the purified monoclonal antibody with RaM FITC conjugate and 100 µl blood sample. However each investigator should titer the antibody in their application to determine the optimal per-test amount. CD4 is also used in studies of functional activity of Th-cells in bacterial and viral infections, development of auto-immune diseases, transplant rejection, immune protection in response to allergens or allergenic reactivity.
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Storage
contact us for more details about storage and/or shipping
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Relevant references
Carrière, D., et al., 1995, In: Leukocyte Typing V: 475-476. S.F. Schlossman, L. Boumsell, W., et al., eds. Oxford University Press, New York Piatier-Tonneau, D., et al.,1995, In: Leukocyte Typing V: 476-478. S.F. Schlossman, L. Boumsell, W. Gilks, et al., eds. Oxford University Press, New York Kaneoka, H. et al., 1983. J. Immunol., 131: 158 Lanier, L.L., et al., 1986. J. Immunol., 137: 2501 Knowles, R.W., 1986. In: Leukocyte Typing II : 259-288; E.L. Reinhert, B.F. Haynes, L.M. Nadler/ and I.D. Bernstein, eds. Springer-Verlag, New York Friedrich, W., 1982. Blood, 59: 696 Allison, J.P. and Lanier, L.L., 1987. Ann. Rev. Immunol., 5: 503
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk. CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
