Blank qPCR Master Mix (2x), plus ROX solution For quantitative real-time PCR and two-step real- time RT-PCR. The master mix does not contain any dye and allows the user to choose a dye and optimize the dye concentration. Contains ROX dye.

• Catalog number
  E0404-02
• Price
  Please ask
• Size
  2X200 reactions

• Group
  PCR, polymerase chain reaction, RT-PCR mixes
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
• Properties
  Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR. Master mixes of Taq polymerases are supplied with dNTP's and Mg+buffer already premixed. They are used for automation and to exclude variaions in your PCR.
• Description
  Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes. The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.

• Gene target
  Blank   qPCR   Master   plus   For   quantitative   two   real   time   The   master   does   not   contain   any   dye   allows   the   user   choose   dye   optimize   the   dye   concentration   Contains   dye  
• Short name
  Blank qPCR Master (2x), plus ROX For quantitative real-time PCR two-step real- time RT-PCR. The master does not contain any dye allows the user choose a dye optimize the dye concentration. Contains ROX dye.
• Technique
  Real-time, RT-PCR, step, PCR, Rox, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. rox labelled 1. steps
• Label
  dye
• Alternative name
  Blank Quantitative real-time PCR test kit Master Mix (2x), plus ROX solution to measure volumetric real-time PCR test kit and two-step real- time Reverse transcription PCR test kit. The master mix does not contain any dye and allows the user to choose a dye and optimize the dye concentration. Contains ROX dye.
• Alternative technique
  rtpcr, rtpcrkits, steps, realtime, dna-amplification

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products