RAD50 Antibody
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Catalog numberbs-11230R
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PricePlease ask
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Size0.1ml
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Long nameRAD50 Primary Polyclonal Antibody
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Also known asRAD50 PAb
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CategoryPrimary Antibodies
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ConjugationUnconjugated
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Target AntigenRAD50
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SpecificityThis is a highly specific antibody against RAD50.
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Modification sNone
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Modification site sUnmodified antibody
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ClonalityPolyclonal antibody
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Clone numberPolyclonal antibody
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Concentration1ug per 1ul
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Immunogen range817-872/1312
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Subcellular locationNucleus
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SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human RAD50
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Gene ID number10111
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Swiss ProtQ92878
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Tested ApplicationsWB
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Recommended dilutionsWB(1:100-1000)
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Cross reactivityHuman, Mouse, Rat
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Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigenComponent of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point (PubMed:11741547, PubMed:9590181, PubMed:9705271, PubMed:9651580). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888).
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym namesDNA repair protein RAD50; RAD50; hRAD50
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PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolRAD50
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Short nameAnti-RAD50
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TechniqueAntibody, antibodies against human proteins, antibodies for
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HostRabbit (Oryctolagus cuniculus)
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IsotypeImmunoglobulin G (IgG)
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Alternative nameRAD50 Polyclonal Antibody
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameRAD50 double strand break repair protein
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Synonyms gene name
- RAD50 (S. cerevisiae) homolog
- RAD50 homolog (S. cerevisiae)
- RAD50 homolog, double strand break repair protein
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-07-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- BRCA1 C complex
- MRN complex
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data