Alternative Names
PPIB; PPI-B; CYP-S1; CyP-B; SCYLP; PPIase B; Rotamase B; S-cyclophilin; Peptidylprolyl Isomerase B; Peptidyl-Prolyl Cis-Trans Isomerase B
Assay Type
Sandwich
Sensitivity
0.33ng/mL
Standard
50ng/mL
Detection range
0.781-50ng/mL
Assay length
3h
Research Area
Tumor immunity;Immune molecule;
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclophilin B (CYPB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclophilin B (CYPB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclophilin B (CYPB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclophilin B (CYPB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus
Gene targetCYPB(Cyclophilin
Short name
CYPB(Cyclophilin B) ELISA Kit
Technique
ELISA kit, ELISA
Host
Rat
Species
Rat
Alternative name
Rat CYPB(Cyclophilin B) Enzyme-linked immunosorbent assay reagent
Alternative technique
elisas
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
