Fructosamine Assay Kit (Colorimetric)
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Catalog number
K450-100
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Price
Please ask
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Size
100 assays
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Description
Simple assay to estimate Fructosamine concentration in biological samples. Validated with human serum
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Summary
• Detection method- Absorbance (530 nm) • Species reactivity- Mammalian
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Detection Method
Absorbance (OD 530 nm)
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Species Reactivity
Mammalian
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Applications
Measurement of Fructosamine levels in serum
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Sample Type
Serum
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Features Benefits
• Simple procedure; takes ~ 1 hour • Fast and convenient • This sensitive kit contains all necessary reagents for accurate measurement of Fructosamine, including a Sample Cleaning Mix that removes matrix interferences
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Fructosamines are stable glycated proteins that are formed by a non-enzymatic reaction between glucose and serum proteins (usually albumin). Elevated concentrations of fructosamine can be found in serum samples of diabetic patients and its detection can be used to assess the glycemic status of diabetics. The half-life of albumin is shorter when compared to hemoglobin (t1/2= 20 days vs. 50 days respectively). Thus, fructosamine levels reflect the efficacy of treatment in diabetic patients during short-term periods, and provide earlier and more sensitive detection for diabetes than many other carbohydrate tests. Biovision’s Fructosamine Assay Kit is a microplate-based colorimetric assay for the direct determination of fructosamine levels in serum. The assay is based on the ability of fructosamine to reduce nitroblue tetrazolium (NBT), forming a colored end-product (purple) under alkaline conditions. The formation rate of formazan is proportional to the concentration of fructosamine in samples and the increase in absorbance (OD 530nm) can be monitored using a spectrophotometer. The kit includes a Thiol Blocking Reagent and a Sample Cleaning Mix that minimizes the interference of other endogenous reducing agents which ensures accurate measurements of Fructosamine in biological samples. The assay is simple, reproducible and can detect as low as 20 µmol/L of fructosamine in samples
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Short name
Fructosamine Assay Kit (Colorimetric)
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Fructosamine test reagent (colorimetry)
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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