Mitochondrial Apoptosis Detection Fluorometric Kit, MitoCapture

• Catalog number
  K250-25
• Price
  Please ask
• Size
  25 assays

• Description
  Distinguishes between apoptotic & healthy cells
• Summary
  • Detection method- Fluorescence microscopy and Flow cytometry: FITC (Ex/Em = 488/530 ± 30 nm); and (optional) PI (Em = 488/590 ± 42 nm) • Species reactivity- Mammalian • Kit size- Convenient sizes (25 and100 assays) • Applications- Disruption of mitochondrial transmembrane potential is one of the earliest intracellular events that occur upon induction of apoptosis. The MitoCapture Mitochondrial Apoptosis Detection Kit provides a simple and sensitive in vitro assay for detecting the mitochondrial changes in apoptosis. It is highly sensive and detects apoptosis in living cells.
• Sample Type
  Live cells
• Features Benefits
  • Simple one-step procedure; takes only 30 minutes • Fast and convenient
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoCapture™ Apoptosis Detection Kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial transmembrane potential. The kit utilizes MitoCapture™, a cationic dye that fluoresces differently in healthy vs apoptotic cells. In healthy cells, MitoCapture accumulates and aggregates in the mitocondria, giving off a bright red fluorescence. In apoptotic cells, MitoCapture cannot aggregate in the mitochondria due to the altered mitochondrial transmembrane potential, and thus it remains in the cytoplasm in its monomer form, fluorescing green. The fluorescent signals can be easily detected by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530+ 30 nm) and (optional) PI channel for red aggregates (Em = 488/590+ 42 nm).
• Data Sheet Link
• Additional description
  This 1 is suited for programmed cell-death studies. The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.

• Gene target
  Mitochondrial   Fluorometric   Kit   MitoCapture  
• Short name
  Mitochondrial Apoptosis Fluorometric Kit, MitoCapture
• Technique
  apoptosis, detection, apoptosis pathway
• Alternative name
  Mitochondrial programmed cellular destruction quantification fluorescence reagent, MitoCapture
• Alternative technique
  celldeath, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  mitochondrial

MeSH Data

• Name
  Radioallergosorbent Test
• Concept
  Scope note: An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.
• Tree numbers
  • E01.370.225.812.735.830
  • E05.200.812.735.830
  • E05.478.566.380.810
  • E05.478.566.639.810
  • E05.478.594.760.830
  • E05.601.470.380.810
  • E05.601.470.639.810
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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