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Category
Primary Antibody
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Antibody Subtype
Conjugated Polyclonal Antibodies
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Area of research
Miscellaneous
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Type of Immunogen
FITC antibody (HRP) was raised in goat using fluorescein conjugated to goat IgG ..
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Raised in
Goat
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Cross Reactivity
Should be determined by the end user
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Conjugate Tag
HRP
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Method of Purification
FITC antibody (HRP) was purified by affinity chromatography.
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Form Buffer
Lyophilized from 0.02M K2PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamycin sulfate. DO NOT add NaN3. I mMunoglobulin and protease free.
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Storage
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
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Shipping conditions
Blue Ice
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Tested for
ELISA; IHC; WB
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Usage Recommendations
ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,000, WB: 1:2,000 - 1:5,000
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
Anti-FITC Antibody, HRP Conjugated
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French translation
anticorps
