Epiregulin (EREG), human recombinant
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Catalog number4069-25
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Price:
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Size25 ug
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SynonymsEpiregulin, EREG
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Alternative_namesEpiregulin, EREG
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DescriptionA ligand of EGFR, and most members of the ERBB family
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥97%
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AssaySDS-PAGE
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Purity≥97%
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Biological activityThe ED₅₀ as dertermined by the dose-dependent stimulation of the proliferation of murine balb/3T3 cells is 5 x 10⁵ units/mg
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Molecular Weight6.0 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionSterile filtered and lyophilized from 0.5 mg/ml solution containing 20 mM PBS, pH 7.4 and 130 mM NaCl.
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Reconstitution InstructionsReconstitute in sterile H₂O to 0.1–1 mg /ml. The solution can then further diluted to other aqueous solutions.
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Background InformationEpiregulin is part of the EGF family. Epiregulin functions as a ligand of EGFR, as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. Epiregulin is expressed mostly in the placenta and peripheral blood leukocytes and in specific carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and Vascular smooth muscle cells. Epiregulin inhibits the growth of several tumor-derived epithelial cell lines. Human Epiregulin is initially synthesized as a glycosylated 19.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a 6.0 kDa mature secreted sequence. Recombinant human EREG produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 6 kDa.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolEREG
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Short nameEpiregulin (EREG), recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameEpiregulin (epiregulin), H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targetepiregulin, ER, EREG and IDBG-24370 and ENSG00000124882 and 2069, growth factor activity, Extracellular, Ereg and IDBG-179047 and ENSMUSG00000029377 and 13874, EREG and IDBG-643034 and ENSBTAG00000010273 and
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Gene info
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Identity
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Gene
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Long gene nameepiregulin
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-02-11
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Entrez gene record
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Pubmed identfication
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data