Epiregulin (EREG), human recombinant

• Catalog number
  4069-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  Epiregulin, EREG
• Alternative_names
  Epiregulin, EREG
• Description
  A ligand of EGFR, and most members of the ERBB family
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥97%
• Assay
  SDS-PAGE
• Purity
  ≥97%
• Biological activity
  The ED₅₀ as dertermined by the dose-dependent stimulation of the proliferation of murine balb/3T3 cells is 5 x 10⁵ units/mg
• Molecular Weight
  6.0 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Sterile filtered and lyophilized from 0.5 mg/ml solution containing 20 mM PBS, pH 7.4 and 130 mM NaCl.
• Reconstitution Instructions
  Reconstitute in sterile H₂O to 0.1–1 mg /ml. The solution can then further diluted to other aqueous solutions.
• Background Information
  Epiregulin is part of the EGF family. Epiregulin functions as a ligand of EGFR, as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. Epiregulin is expressed mostly in the placenta and peripheral blood leukocytes and in specific carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and Vascular smooth muscle cells. Epiregulin inhibits the growth of several tumor-derived epithelial cell lines. Human Epiregulin is initially synthesized as a glycosylated 19.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a 6.0 kDa mature secreted sequence. Recombinant human EREG produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 6 kDa.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Epiregulin   EREG  
• Gene symbol
  EREG
• Short name
  Epiregulin (EREG), recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Epiregulin (epiregulin), H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  epiregulin, ER, EREG and IDBG-24370 and ENSG00000124882 and 2069, growth factor activity, Extracellular, Ereg and IDBG-179047 and ENSMUSG00000029377 and 13874, EREG and IDBG-643034 and ENSBTAG00000010273 and

Gene info

• Identity
  HGNC:3443
• Gene
  EREG
• Long gene name
  epiregulin
• Synonyms
  • ER
• GenBank acession
  • D30783
• Locus
  4q13.3
• Discovery year
  1998-02-11
• Entrez gene record
  2069
• Pubmed identfication
  • 9337852
• VEGA ID
  OTTHUMG00000130005

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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