Mouse Anti-Mouse REEP1 Monoclonal IgG2B, Clone S345-51
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Catalog numberSMC-480D-ALP
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PricePlease ask
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Size100 µg
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CloneS345-51
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ImmunogenFusion protein amino acids 111-201 of mouse REEP1
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Antibody s full descriptionMouse Anti-Mouse REEP1 Monoclonal IgG2B Antibody, Clone: S345-51: Alkaline Phosphatase
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameReceptor expression-enhancing protein 1 Antibody, C2orf23 Antibody, D6Ertd253e Antibody, rcG_56072 Antibody, Reep1 Antibody, SPG31 Antibody
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Raised inMouse
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Antibody s targetREEP1
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Primary research fieldsNeuroscience, Neurodegeneration, Spastic Paraplegia, Cell Structure, Membrane Markers
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~22kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisA 1:100 dilution of SMC-480 was sufficient for detection of REEP1 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMitochondrion , Mitochondrion Membrane , Endoplasmic Reticulum , Endoplasmic Reticulum Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextREEP1 (receptor expression-enhancing protein 1), is a 201 amino acid multi-pass mitochondrion membrane protein that belongs to the DP1 family. REEP1 interacts with odorant receptor proteins and may enhance the cell surface expression of odorant receptors (1, 2). It is also required for endoplasmic-reticulum network formation shaping and remodeling by linking ER tubules to the cytoskeleton (2). Mutations in the REEP1 gene are a common cause of hereditary spastic paraplegia (HSP). Mutations in the REEP1 gene also cause spastic paraplegia autosomal dominant type 31, a neurodegenerative disorder (3).
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Bibliography
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Released date10-Mar-2015
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_848723.1
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Gene number52250
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Protein numberQ8BGH4
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-REEP1 Monoclonal Antibody, Clone S345-51 (SMC-480). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-REEP1 Monoclonal Antibody (SMC-480) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) REEP1 Antibody (D) Composite. Western Blot analysis of Rat Brain showing detection of ~22 kDa REEP1 protein using Mouse Anti-REEP1 Monoclonal Antibody, Clone S345-51 (SMC-480). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-REEP1 Monoclonal Antibody (SMC-480) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~22 kDa. Mouse Anti-REEP1 Antibody [S345-51] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-480) Mouse Anti-REEP1 Antibody [S345-51] used in Western Blot (WB) on Rat Brain (SMC-480)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolIGHV8-51-1, IGHVII-51-2, IGLV1-51, IGHV5-51, REEP1, ZNF3, SAMM50, OR7E26P
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Short nameMouse Anti-Mouse REEP1 Monoclonal IgG2B, Clone S345-51
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2B, IgG2b
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LabelAlkaline Phosphatase
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse REEP1 monoclonal IgG2B, clonality S345-51
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Alternative techniquemurine, antibodies
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Clone nameS345-51
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable IGHV8-51-1 (non-functional)
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable (III)-51-1
- immunoglobulin heavy variable (III)-51-1 pseudogene
- immunoglobulin heavy variable (III)-51-1 (pseudogene)
- immunoglobulin heavy variable IGHV8-51-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-51-2 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-51-2
- immunoglobulin heavy variable (II)-51-2 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 1-51
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 5-51
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namereceptor accessory protein 1
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Synonyms gene
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Synonyms gene name
- chromosome 2 open reading frame 23
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-04-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor accessory proteins
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namezinc finger protein 3
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Synonyms gene name
- zinc finger protein 3 (A8-51)
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-05-31
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Entrez gene record
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSAMM50 sorting and assembly machinery component
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Synonyms gene name
- sorting and assembly machinery component 50 homolog (S. cerevisiae)
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-05-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitochondrial sorting and assembly machinery complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 7 subfamily E member 26 pseudogene
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Synonyms gene
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Synonyms gene name
- olfactory receptor, family 7, subfamily E, member 26 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-11-29
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Entrez gene record
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Pubmed identfication
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Classification
- Olfactory receptors, family 7
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data