Mouse Anti-Mouse REEP1 Monoclonal IgG2B, Clone S345-51

• Catalog number
  SMC-480D-A488
• Price
  Please ask
• Size
  100 µg

• Clone
  S345-51
• Immunogen
  Fusion protein amino acids 111-201 of mouse REEP1
• Antibody s full description
  Mouse Anti-Mouse REEP1 Monoclonal IgG2B Antibody, Clone: S345-51: ATTO 488
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  Receptor expression-enhancing protein 1 Antibody, C2orf23 Antibody, D6Ertd253e Antibody, rcG_56072 Antibody, Reep1 Antibody, SPG31 Antibody
• Raised in
  Mouse
• Antibody s target
  REEP1
• Primary research fields
  Neuroscience, Neurodegeneration, Spastic Paraplegia, Cell Structure, Membrane Markers
• Brandname
  None
• Antibodies applications
  WB, IHC, ICC/IF
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.1% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~22kDa.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  A 1:100 dilution of SMC-480 was sufficient for detection of REEP1 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Mitochondrion , Mitochondrion Membrane , Endoplasmic Reticulum , Endoplasmic Reticulum Membrane
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  REEP1 (receptor expression-enhancing protein 1), is a 201 amino acid multi-pass mitochondrion membrane protein that belongs to the DP1 family. REEP1 interacts with odorant receptor proteins and may enhance the cell surface expression of odorant receptors (1, 2). It is also required for endoplasmic-reticulum network formation shaping and remodeling by linking ER tubules to the cytoskeleton (2). Mutations in the REEP1 gene are a common cause of hereditary spastic paraplegia (HSP). Mutations in the REEP1 gene also cause spastic paraplegia autosomal dominant type 31, a neurodegenerative disorder (3).
• Bibliography
  1. Genes and mapped phenotypes. (n.d.). Retrieved March 11, 2015, from more 2. Park S.H., Zhu P.P., Parker R.L. Blackstone C. (2010) J Clin Invest. 120(4): 1097-1110. 3. REEP1 - Receptor expression-enhancing protein 1 - Homo sapiens (Human). (n.d.). Retrieved March 11, 2015, from details
• Released date
  10-Mar-2015
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_848723.1
• Gene number
  52250
• Protein number
  Q8BGH4
• Antibody s datasheet
  Contact our support service
• Representative figure link
  see here | read more
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-REEP1 Monoclonal Antibody, Clone S345-51 (SMC-480). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-REEP1 Monoclonal Antibody (SMC-480) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) REEP1 Antibody (D) Composite. Western Blot analysis of Rat Brain showing detection of ~22 kDa REEP1 protein using Mouse Anti-REEP1 Monoclonal Antibody, Clone S345-51 (SMC-480). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-REEP1 Monoclonal Antibody (SMC-480) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~22 kDa. Mouse Anti-REEP1 Antibody [S345-51] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-480) Mouse Anti-REEP1 Antibody [S345-51] used in Western Blot (WB) on Rat Brain (SMC-480)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  REEP1   Monoclonal   IgG2B   S345-51  
• Gene symbol
  IGHV8-51-1, IGHVII-51-2, IGLV1-51, IGHV5-51, REEP1, ZNF3, SAMM50, OR7E26P
• Short name
  Mouse Anti-Mouse REEP1 Monoclonal IgG2B, Clone S345-51
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG2B, IgG2b
• Label
  ATTO 488
• Species
  Mouse, Mouses
• Alternative name
  Mouse Antibody toMouse REEP1 monoclonal IgG2B, clonality S345-51
• Alternative technique
  murine, antibodies
• Clone name
  S345-51

Gene info

• Identity
  HGNC:5704
• Gene
  IGHV8-51-1
• Long gene name
  immunoglobulin heavy variable IGHV8-51-1 (non-functional)
• Synonyms gene
  • IGHVIII-51-1
• Synonyms gene name
  • immunoglobulin heavy variable (III)-51-1
  • immunoglobulin heavy variable (III)-51-1 pseudogene
  • immunoglobulin heavy variable (III)-51-1 (pseudogene)
  • immunoglobulin heavy variable IGHV8-51-1 (pseudogene)
• Synonyms
  • IGHV(III)-51-1
• GenBank acession
  • AB019438
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28343
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151957

Gene info

• Identity
  HGNC:5684
• Gene
  IGHVII-51-2
• Long gene name
  immunoglobulin heavy variable (II)-51-2 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-51-2
  • immunoglobulin heavy variable (II)-51-2 pseudogene
• Synonyms
  • IGHV(II)-51-2
• GenBank acession
  • AB019438
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28363
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151969

Gene info

• Identity
  HGNC:5882
• Gene
  IGLV1-51
• Long gene name
  immunoglobulin lambda variable 1-51
• GenBank acession
  • Z73661
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28820
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151035

Gene info

• Identity
  HGNC:5659
• Gene
  IGHV5-51
• Long gene name
  immunoglobulin heavy variable 5-51
• GenBank acession
  • M99686
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28388
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151960

Gene info

• Identity
  HGNC:25786
• Gene
  REEP1
• Long gene name
  receptor accessory protein 1
• Synonyms gene
  • C2orf23
• Synonyms gene name
  • chromosome 2 open reading frame 23
• Synonyms
  • FLJ13110
  • SPG31
  • Yip2a
• Synonyms name
  • receptor expression enhancing protein 1
• GenBank acession
  • AK023172
• Locus
  2p11.2
• Discovery year
  2004-04-27
• Entrez gene record
  65055
• Pubmed identfication
  • 16271481
  • 15550249
• RefSeq identity
  • NM_022912
• Classification
  • Receptor accessory proteins
• VEGA ID
  OTTHUMG00000130205
• Locus Specific Databases
  • LRG_713

Gene info

• Identity
  HGNC:13089
• Gene
  ZNF3
• Long gene name
  zinc finger protein 3
• Synonyms gene name
  • zinc finger protein 3 (A8-51)
• Synonyms
  • A8-51
  • KOX25
  • PP838
  • FLJ20216
  • HF.12
  • Zfp113
• GenBank acession
  • AF027136
• Locus
  7q22.1
• Discovery year
  1989-05-31
• Entrez gene record
  7551
• RefSeq identity
  • NM_017715
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000154596

Gene info

• Identity
  HGNC:24276
• Gene
  SAMM50
• Long gene name
  SAMM50 sorting and assembly machinery component
• Synonyms gene name
  • sorting and assembly machinery component 50 homolog (S. cerevisiae)
• Synonyms
  • CGI-51
  • TRG-3
  • YNL026W
  • OMP85
  • TOB55
  • SAM50
• GenBank acession
  • AK001087
• Locus
  22q13.31
• Discovery year
  2005-05-31
• Entrez gene record
  25813
• Pubmed identfication
  • 15644312
• RefSeq identity
  • NM_015380
• Classification
  • Mitochondrial sorting and assembly machinery complex
• VEGA ID
  OTTHUMG00000150557

Gene info

• Identity
  HGNC:8398
• Gene
  OR7E26P
• Long gene name
  olfactory receptor family 7 subfamily E member 26 pseudogene
• Synonyms gene
  • OR7E67P
  • OR7E69P
  • OR7E70P
  • OR7E68P
• Synonyms gene name
  • olfactory receptor, family 7, subfamily E, member 26 pseudogene
• Synonyms
  • OR1-51
  • OR1-72
  • OR1-73
  • OR912-95
• GenBank acession
  • U86301
• Locus
  10p13
• Discovery year
  1999-11-29
• Entrez gene record
  401637
• Pubmed identfication
  • 9500546
• Classification
  • Olfactory receptors, family 7
• VEGA ID
  OTTHUMG00000017721

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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