TDGF1, human recombinant
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Catalog number4335-1000
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PricePlease ask
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Size1000 ug
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SynonymsCRIPTO1, CRIPTO-1, CRGF, Teratocarcinoma-Derived Growth Factor 1, TDGF1, CR1.
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Alternative_namesCRIPTO1, CRIPTO-1, CRGF, Teratocarcinoma-Derived Growth Factor 1, TDGF1, CR1.
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DescriptionA signaling protein involved in embryonic development and tumor growth
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RecombinantYes
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SourceHuman cells
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Biological activityMeasured by its binding ability in a functional ELISA Immobilized human TDGF1 at 2 ug/ml ( 100 ul/well) can bind human ALK4 with a linear range of 0.0068 - 0.16 ug/ml.
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Molecular Weight17.4 kDa
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Storage Temp-70°C
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Shippinggel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionLyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
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Reconstitution InstructionsReconstitute in sterile PBS
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Background InformationTeratocarcinoma-derived growth factor 1, also known as epidermal growth factor-like cripto protein CR1, CRGF, and TDGF1, is a cell membrane which contains one EGF-like domain. In humans, TDGF1 is highly expressed in germ cell tumors and in colon and mammary carcinomas. TDGF1 is a member of the epidermal growth factor-cripto FRL1 cryptic protein family and is involved in the activation of several different signaling pathways during embryonic development and cellular transformation. TDGF1 regulates the activation of several signaling pathways and controls cellular transformation in embryonic status. Patients with high TDGF1 expression were statistically susceptible to a recurrence of the disease, and showed poorer disease-free survival than those with low expression. TDGF1 is a predictive marker for metachronous metastasis in colorectal cancer (CRC) patients. It is preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. TDGF1 plays a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
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Amino acid sequenceA DNA sequence encoding the human TDGF1 (AAH22393.1) (Met 1 - Thr 172) with a C-terminal polyhistidine tag was expressed.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolTDGF1
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Short nameTDGF1, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameteratocarcinoma-derived growth factor 1, H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targetteratocarcinoma-derived growth factor 1, CR and CRGF and CRIPTO, TDGF1 and IDBG-409017 and ENSG00000241186 and 6997, growth factor activity, nuclei, Tdgf1 and IDBG-202568 and ENSMUSG00000032494 and 21667
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Gene info
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Identity
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Gene
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Long gene nameteratocarcinoma-derived growth factor 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-08-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data