ALPL Polyclonal Antibody, FITC Conjugated
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Catalog number
A53200-050
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Price
Please ask
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Size
50 µg
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Note
For research use only.
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Properties
If you buy Antibodies supplied by EpiGentek they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This EpiGentek Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. EpiGentek FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
ALPL
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Short name
ALPL Polyclonal Antibody, FITC Conjugated
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Technique
Polyclonal, Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Label
FITC
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Alternative name
alkaline phosphatase, liver/bone/kidney polyclonal (antibody to-), fluorecein coupled
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Alternative technique
polyclonals, antibodies, fluorescine
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Alternative to gene target
alkaline phosphatase, liver/bone/kidney, AP-TNAP and APTNAP and HOPS and TNAP and TNSALP, ALPL and IDBG-93194 and ENSG00000162551 and 249, metal ion binding, Extracellular, Alpl and IDBG-199139 and ENSMUSG00000028766 and 11647, ALPL and IDBG-645614 and ENSBTAG00000008951 and 280994
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Gene info
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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