SMARCA4 Antibody

• Catalog number
  6156-100
• Price
  Please ask
• Size
  100 μg

• Antibody Target
  SMARCA4
• Target Alternative Name
  BAF190, BRG1, FLJ39786, SNF2, SNF2­BETA, SNF2L4, SNF2LB, SWI2, hSNF2b.
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  A synthetic peptide of human SMARCA4
• Accession
  P51532
• Gene Id
  6597
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  1 mg/ml
• Formulation
  100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
• Purification
  Affinity purified
• Application
  Western blot, Immunohistochemistry
• Positive Control
  Western Blot: Various cell lysates
• Application And Usages
  Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200.
• Description
  The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone­DNA contacts by ATP­dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP­dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM. This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant. SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle and differentiation. Brg1/hBRM has been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors. Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression including with HDACs, Rb and Tif1β. Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also plays a role as a tumor suppressors and Brg1 is mutated in several tumor cell lines.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  SMARCA4  
• Gene symbol
  SMARCA4
• Short name
  SMARCA4 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  SMARCA4 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:11100
• Gene
  SMARCA4
• Long gene name
  SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Synonyms gene
  • SNF2L4
• Synonyms
  • hSNF2b
  • BRG1
  • BAF190
  • SNF2
  • SWI2
  • SNF2-BETA
  • SNF2LB
  • FLJ39786
• Synonyms name
  • SNF2-like 4
  • global transcription activator homologous sequence
  • sucrose nonfermenting-like 4
  • mitotic growth and transcription activator
  • BRM/SWI2-related gene 1
  • homeotic gene regulator
  • nuclear protein GRB1
  • brahma protein-like 1
  • ATP-dependent helicase SMARCA4
• GenBank acession
  • D26156
• Locus
  19p13.2
• Discovery year
  1995-07-17
• Entrez gene record
  6597
• Pubmed identfication
  • 8208605
• RefSeq identity
  • NM_003072
• Classification
  • Bromodomain containing
  • PBAF complex
  • BAF complex
  • GBAF complex
• VEGA ID
  OTTHUMG00000169272
• Locus Specific Databases
  • LRG_878

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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