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Alternative name1
Anti-Rabbit anti Tritirachium album Proteinase K, conjugated with Biotin
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Alternative name2
Rabbit anti Tritirachium album Proteinase K, conjugated with Biotin
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Alternative name3
Lapin anti Tritirachium album Proteinase K, conjugated with Biotin
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Alternative name4
Tritirachium album Proteinase K
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Alternative name5
N/A
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Other names
N/A
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Category
Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
N/A
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Clone
Polyclonal antibody
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Host organism
Rabbit
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Species reactivity
Cross-reactivities against enzymes of other sources may occur but have not been determined.
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Specificity and cross reactivity
Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to proteinase K from Tritirachium album.
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Purification method
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
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Form Appearance
Biotin-coupled hyperimmune rabbit IgG Iyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
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Concentration
N/A
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Storage and shipping
Store at Store productone at +4 degrees Celsius.. Handling:The Iyophilised conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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Tested for
ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Gene
Proteinase K s a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa).
