Phospho-Raf Antibody
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Catalog number3504-100
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PricePlease ask
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Size100 µg
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Antibody TargetPhospho-Raf (THR259)
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Target Alternative NameRAF1 , cRaf , Raf-1 , c-Raf , RAF , CRAF , C-RAF, RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceSynthetic phospho-peptide surrounding amino acid Ser259 of human c-Raf
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AccessionP04049
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Gene Id5894
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation100 µg (0.5 mg/ml) affinity purified rabbit anti-Phospho-Raf (Ser259) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot, Immunohistochemistry
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Application And UsagesWestern blotting (0.5-4 µg/ml) and Immunohistochemistry (20-40µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~74 kDa c-Raf when phosphorylated at Ser259.
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Descriptionc-Raf is a mitogen-activated protein kinase. Binding of Raf to 14-3-3 is required for c-Raf kinase activity, and also blocks the ability of phosphatase to inhibit c-Raf. 14-3-3 binds c-Raf at two phospho-serine residues: Ser259 and Ser621. Mutation of Ser259 to alanine constitutively activates Raf kinase activity, but mutation of Ser621 to alanine results in an inactive kinase.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Short namePhospho-Raf Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative namephosphorilated-Raf (antibody to-)
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data