CD144 / VE-cadherin monoclonal antibody
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Catalog number1P-770-T100
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PricePlease ask
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Size100 assays
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Long nameMouse Monoclonal Antibody against Human CD144 / VE-cadherin, Clone: 55-7H1
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AntigenCD144
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Species reactivityHuman (Homo sapiens)
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Negative species reactivityat the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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ClonalityMonoclonal Antibody (Mab)
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Clone55-7H1
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ApplicationsFC, IP, WB, IHC(F), ICC
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ImmunogenHuman endothelial cells
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Format conjugationPE
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SpecificityThe mouse monoclonal antibody 55-7H1 recognizes a calcium-independent epitope on CD144 (VE-cadherin, cadherin 5), an adhesion molecule expressed on endothelial cells.
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Research areaCD and Related Antigens (Human)
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Storage bufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
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Shipping and storageMouse Monoclonal Antibody against Human CD144 / VE-cadherin, Clone: 55-7H1 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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BackgroundCD144 / VE-cadherin (cadherin 5) is the major cadherin that is present at endothelial junctions. It is also strictly endothelial specific. Under vascular permeability increasing conditions (and also in capillaries and veins) CD144 is being phosphorylated, which promotes its rapid and reversible internalization. On the contrary, binding of p120 catenin (delta1 catenin) maintains CD144 localization at the plasma membrane, which stabilizes the junction and reduces vascular permeability.
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Purity> 95% (by SDS-PAGE)
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Purification methodPurified by affinity chromatography
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References*Tisato V, Zauli G, Voltan R, Gianesini S, di Iasio MG, Volpi I, Fiorentini G, Zamboni P, Secchiero P: Endothelial cells obtained from patients affected by chronic venous disease exhibit a pro-inflammatory phenotype. PLoS One. 2012;7(6):e39543., *Agrawal V, Kelly J, Tottey S, Daly KA, Johnson SA, Siu BF, Reing J, Badylak SF: An isolated cryptic peptide influences osteogenesis and bone remodeling in an adult mammalian model of digit amputation. Tissue Eng Part A. 2011 Dec;17(23-24):3033-44., *De Ugarte DA, Alfonso Z, Zuk PA, Elbarbary A, Zhu M, Ashjian P, Benhaim P, Hedrick MH, Fraser JK: Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow. Immunol Lett. 2003 Oct 31;89(2-3):267-70., *Limaye V, Li X, Hahn C, Xia P, Berndt MC, Vadas MA, Gamble JR: Sphingosine kinase-1 enhances endothelial cell survival through a PECAM-1-dependent activation of PI-3K/Akt and regulation of Bcl-2 family members. Blood. 2005 Apr 15;105(8):3169-77, *Hamilton RD, Foss AJ, Leach L: Establishment of a human in vitro model of the outer blood-retinal barrier. J Anat. 2007 Dec;211(6):707-16
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PropertiesIf you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translationanticorps
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Gene target
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Gene symbolJAM2, CDH5
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Short nameAnti-CD144 / VE-cadherin Mab
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TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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HostMouse (Mus musculus)
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IsotypeIgG1
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Alternative nameMouse Monoclonal to CD144 / VE-cadherin
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namejunctional adhesion molecule 2
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-04-25
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Entrez gene record
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Pubmed identfication
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Classification
- CD molecules
- Ig-like cell adhesion molecule family
- I-set domain containing
- V-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecadherin 5
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Synonyms gene name
- cadherin 5, type 2, VE-cadherin (vascular epithelium)
- cadherin 5, type 2 (vascular endothelium)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1992-11-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Type II classical cadherins
- CD molecules
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data