Mouse Anti-Myeloperoxidase (MPO)
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Catalog number
GEN570363
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Price
Please ask
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Size
200ug
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Also known as
Myeloperoxidase
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Other names
Myeloperoxidase; Myeloperoxidase; myeloperoxidase; myeloperoxidase; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
MPO; MPO; N/A; MPO
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Gene name synonims
MPO; MPO; N/A; MPO
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Other gene names
MPO; MPO; N/A; MPO
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Clonality
Monoclonal antibody
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Immunoglobulin isotype
IgG1
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Clone
8000000
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Host organism
Mouse (Mus musculus)
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Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
The MPO-C2 mAb (clone 8E2) reacts with human myeloperoxidase (MPO) expressed by normal and malignant myelomonocytic cells. The sensitivity of MPO-C2 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb-concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ul of leukocytes containing 10^7 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity. ; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
Purified by Chromatography, Storage buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3
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Concentration
N/A
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Storage and shipping
the antibody contains monoclonal antibody specific reagents in optimal concentrations of affinity-purified antibody. To enchance the antibody's stability sodium azide has been added. Keep the antibody at +4 degrees Celsius and do NOT freeze it. the antibody may be photosensitive, therefore avoid exposing the vial to direct bright light. Please refer to the expiry date printed on the vial. The use of the reagent after the expiration date is not recommended.
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Tested applications
Flow Cytometry (FC/FACS)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
MPO
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Short name
Mouse Anti-Myeloperoxidase (MPO)
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Technique
anti-, Mouse, anti, antibody to, antibodies, mouses
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Host
mouse
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Species
Mouse, Mouses
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Alternative name
Anti-Mouse anti Myeloperoxidase (MPO)
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Alternative technique
murine, antibodies
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Alternative to gene target
myeloperoxidase, MPO and IDBG-60932 and ENSG00000005381 and 4353, metal ion binding, nuclei, Mpo and IDBG-207719 and ENSMUSG00000009350 and 17523, MPO and IDBG-629390 and ENSBTAG00000011616 and 511206
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Gene info
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Identity
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Gene
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Long gene name
myeloperoxidase
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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