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Products_type
Secondary Antibody
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Products_short_name
[IgM (u chain)]
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Products_name_syn
[Goat anti-Mouse IgM (u chain) - Affinity Pure]
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Other_names
[immunoglobulin mu chain, partial; Ig heavy chain Mem5-like]
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Other_gene_names
[LOC641025; Igh; Igm; IGHV; IgVH; CRP55H]
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Reactivity
Mouse. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin mouse serum proteins light chains on all mouse immunoglobulins
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Specificity
Based on IEP, this antibody reacts with: heavy chains on mouse IgM
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Purity
Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Mouse IgM.
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Form
Clear, fluorescent yellow liquid
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Concentration
1.50 mg/ml (E 1% at 280 nm = 13.0)
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Storage_stability
Store at 2-8 degree C. Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Tested_application
Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM)
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Test
A high affinity purification column was use to purify Goat anti-Mouse IgM (u chain) - Pure, FITC Conjugate by MyBioSource by chromatographic size exclusion. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure Goat anti-Mouse IgM (u chain) - Pure, FITC Conjugate supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus, Mus musculus
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Gene target
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Short name
Goat anti-Mouse IgM (u chain) - Pure, FITC Conjugate
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Technique
affinity, anti-, Mouse, anti, FITC, Goat, Pure, IgM, antibody to, antibodies, Fluorescein, goats, IgMs, mouses, pure products
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgM, IgM
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Label
FITC
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Species
Mouse, Goats, Mouses
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Alternative name
caprine Antibody toMouse Immunoglobulin M (u epitope) - protein Pure, fluorecein Conjugate
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Alternative technique
murine, antibodies, purified, caprine, fluorescine, immunoglobulin-m
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