Recombinant Human Ficolin-1 / Ficolin-A / FCN1 Protein (His tag) 100µg
-
Catalog number
PKSH031350-100µg
-
Price
Please ask
-
Size
100µg
-
-
Synonym
FCN1;FCNA;FCNM
-
Activity
Measured by its binding ability in a functional ELISA.Immobilized FCN1 at 10 μg/ml can bind biotinylated recombinant human Fetuin-A with a linear range of 16-2000 ng/ml.2. Immobilized FCN1 at 10 μg/ml can bind biotinylated recombinant mouse Fetuin-A with a linear range of 16-2000 ng/ml.
-
Sequence
Met 1-Ala 326
-
Fusion tag
C-His
-
Accession
NP_001994.2
-
Expressed Host
HEK293 Cells
-
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
-
Purity
>95 % as determined by SDS-PAGE
-
Endotoxin
<1.0 EU per µg as determined by the LAL method
-
Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Mol Mass
33.6 kDa
-
AP Mol Mass
33.6 kDa
-
Formulation
Lyophilized from sterile PBS, pH 7.4
-
Reconstitution
Please refer to it for detailed information.
-
Background
Ficolins are humoral molecules of the innate immune systems which recognize carbohydrate molecules on pathogens, apoptotic and necrotic cells. The Ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins are humoral molecules of the innate immune systems which recognize carbohydrate molecules on pathogens, apoptotic and necrotic cells. Three Ficolins have been identified in humans: L-Ficolin, H-Ficolin and M-Ficolin (also referred to as Ficolin-2, -3 and -1, respectively). They are soluble oligomeric defence proteins with lectin-like activity and they are structurally similar to the human collectins, mannan-binding lectin (MBL) and surfactant protein A and D. Dysfunction or abnormal expressions of Ficolins may involved in the pathogenesis of human diseases including infectious and inflammatory diseases, autoimmune disease and clinical syndrome of preeclampsia. They are soluble oligomeric defence proteins with lectin-like activity and they are structurally similar to the human collectins, mannan-binding lectin (MBL) and surfactant protein A and D. Upon recognition of the infectious agent, the Ficolins act through two distinct routes: initiate the lectin pathway of complement activation through attached serine proteases (MASPs), and a primitive opsonophagocytosis thus limiting the infection and concurrently orchestrating the subsequent adaptive clonal immune response. Ficolin-1 (FCN1) is predominantly expressed in the peripheral blood leukocytes.
-
-
-
Conjugation
histidine
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
FCN1
-
Short name
Recombinant Ficolin-1 / Ficolin-A / FCN1 Protein (His tag) 100µg
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
-
Label
His
-
Species
Human, Humans
-
Alternative name
Rec. H. sapiens Ficolin-1 / Ficolin-A / ficolin (collagen/fibrinogen domain containing) 1 Protein (histidine detection labelled) 100µg
-
Alternative technique
rec
-
Alternative to gene target
ficolin (collagen/fibrinogen domain containing) 1, FCNM, FCN1 and IDBG-91827 and ENSG00000085265 and 2219, carbohydrate binding, Extracellular
-
Gene info
-
Identity
-
Gene
-
Long gene name
ficolin 1
-
Synonyms gene name
- ficolin (collagen/fibrinogen domain-containing) 1
- ficolin (collagen/fibrinogen domain containing) 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year
1996-07-11
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Fibrinogen C domain containing
- Complement system activation components
-
VEGA ID
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products