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Clone
S147-6
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Immunogen
Fusion protein amino acids 1-341 (full-length) of human QKI-5. Mouse: 100% identity (341/341 amino acids identical). Rat: 99% identity (339/341 amino acids identical) >90% identity with QKI-6, QKI-7 and QKI-7b.
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Antibody s full description
Mouse Anti-Human QKI Monoclonal IgG2b Antibody, Clone: S147-6: ATTO 700
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
Protein quaking Antibody, Hqk Antibody, HqkI Antibody, QKI Antibody, KH Domain Containing Antibody, RNA Binding (QKI) Antibody
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Raised in
Mouse
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Antibody s target
QKI
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Primary research fields
Neuroscience, Neurodegeneration, Cell Signaling, Scaffolds
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH 7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~36-38kDa.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-467 was sufficient for detection of Pan-QKI in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm , Nucleus
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
QKI is also called Protein Quaking or HqkI. QKI is an RNA-binding protein that plays a central role in myelinization. QKI acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation, and is itself, regulated by alternative splicing. QKI is expressed in the frontal cortex of brain, but is shown to be down-regulated in the brain of schizophrenic patients.
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Released date
26-Sep-2013
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_006766.1.
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Gene number
9444
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Protein number
Q96PU8
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-QKI (pan) Monoclonal Antibody, Clone S147-6 (SMC-467). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-QKI (pan) Monoclonal Antibody (SMC-467) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) QKI (pan) Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of 36-38 kDa QKI (pan) protein using Mouse Anti-QKI (pan) Monoclonal Antibody, Clone S147-6 (SMC-467). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-QKI (pan) Monoclonal Antibody (SMC-467) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 36-38 kDa. Mouse Anti-QKI (pan) Antibody [S147-6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-467) Mouse Anti-QKI (pan) Antibody [S147-6] used in Western Blot (WB) on Rat Brain Membrane (SMC-467)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
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Gene target
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Gene symbol
QKI, MIR1302-6, PIRC28, PIRC24, PIRC26, PIRC27, PIRC25, ADCP1
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Short name
Mouse Anti- QKI (pan) Monoclonal IgG2b, Clone S147-6
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Technique
anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Host
mouse
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Isotype
IgG2b, IgG2b
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Label
ATTO 700
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Species
Human, Humans, Mouses
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Alternative name
Mouse Antibody toHuman QKI (pan) monoclonal IgG2b, clonality S147-6
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Alternative technique
murine, antibodies
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Clone name
S147-6
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