Dnmt2 Antibody
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Catalog number3488-100
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PricePlease ask
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Size100 µg
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Antibody TargetDNMT2
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Target Alternative NameDnmt2 , TRDMT1 , RNMT1 , OTTHUMP00000045198 , cytosine-5-methyltransferase , EC 2.1.1.29
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse
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Immunogen SequenceSynthetic peptides surrounding amino acid 42 and 364 of mouse Dnmt2
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AccessionO55055
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Gene Id13434
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot, Immunofluorescence
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Application And UsagesWestern blotting (0.5-4 µg/ml) and immunofluorescence. However, the optimal concentrations should be determined individually. The antibody recognizes 42 kDa Dnmt 2 of human and mouse origins. Mouse testes tissue lysate can be use as a positive control.
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DescriptionMethylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolTRDMT1
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Short nameDnmt2 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameDnmt2 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nametRNA aspartic acid methyltransferase 1
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Synonyms gene
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Synonyms gene name
- DNA (cytosine-5-)-methyltransferase 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-05-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 7BS C5-cytosine DNA/RNA methyltransferases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data