Profilin 1, Human Recombinant

• Catalog number
  P1365-50
• Price
  Please ask
• Size
  50 µg

• Synonyms
  Epididymis tissue protein Li 184a, PFN1
• Alternative_names
  Epididymis tissue protein Li 184a, PFN1
• Description
  Ubiquitous actin monomer-binding protein
• Recombinant
  Yes
• Source
  E.coli
• Purity by SDS PAGE
  >95%
• Assay
  SDS-PAGE
• Molecular Weight
  15.0 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  In 20 mM Tris-HCl buffer (pH8.0) containing, 10% glycerol
• Background Information
  Profilin1 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein significantly enhances skin wound healing in-vitro and in-vivo that may be mediated by purinergic receptors. It is also active in endothelial cell migration and vessel sprouting. It is thought to regulate actin polymerization in response to extracellular signals. Recombinant Profilin1 protein was expressed in E.coli and purified by using conventional chromatography techniques
• Amino acid sequence
  MAGWNAYIDN LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VNITPAEVGV LVGKDRSSFY VNGLTLGGQK CSVIRDSLLQ DGEFSMDLRT KSTGGAPTFN VTVTKTDKTL VLLMGKEGVH GGLINKKCYE MASHLRRSQY
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used on humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Profilin  
• Gene symbol
  PFN1P6, PFN1, PFN1P4
• Short name
  Profilin 1, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Profilin 1, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:42988
• Gene
  PFN1P6
• Long gene name
  profilin 1 pseudogene 6
• Synonyms gene
  • PFN1P7
• Synonyms gene name
  • profilin 1 pseudogene 7
• Locus
  1q21.1
• Discovery year
  2011-09-16
• Entrez gene record
  645126
• RefSeq identity
  • NG_005762
• VEGA ID
  OTTHUMG00000013834

Gene info

• Identity
  HGNC:8881
• Gene
  PFN1
• Long gene name
  profilin 1
• GenBank acession
  • BC057828
• Locus
  17p13.2
• Discovery year
  1988-08-15
• Entrez gene record
  5216
• Pubmed identfication
  • 3356709
  • 1968707
• RefSeq identity
  • NM_005022
• Classification
  • Profilins
• VEGA ID
  OTTHUMG00000099396

Gene info

• Identity
  HGNC:42992
• Gene
  PFN1P4
• Long gene name
  profilin 1 pseudogene 4
• Synonyms gene
  • PFN1P5
• Synonyms gene name
  • profilin 1 pseudogene 5
• Locus
  1q21.2
• Discovery year
  2012-02-29
• Entrez gene record
  767851
• VEGA ID
  OTTHUMG00000043429

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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