SMAD2/3 (Acetyl-K19) polyclonal antibody

• Catalog number
  BS64092-50ul
• Price
  Please ask
• Size
  50ul

• Alternative Name
  Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; MADH3
• Immunogen
  Synthetic peptide derived from humanSMAD2/3 around the acetylated at Lys19.
• Swiss Prot
  Q15796/P84022
• Reactivity
  Human,Mouse,Rat
• Applications
  WB
• Application_all
  WB: 1:500~1:1000
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  SMAD2/3 (Acetyl-K19) polyclonal antibody detects endogenous levels of SMAD2/3 protein only when acetylated at Lys19.
• Usage
  Western blot (WB) analysis of SMAD2/3 (Acetyl-K19) polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:NIH-3T3 whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug)
• BiowMW
  ~ 89 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Description
  N terminal acetylation or CH3CO as epigenetic regulation of SMAD2/3 ( -K19) polyclonal antibody by NATs.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  SMAD2/3   K19  
• Gene symbol
  SMAD2, KRT19
• Short name
  SMAD2/3 ( -K19) polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit
• Alternative name
  SMAD family member 2/3 (Acetyl-K19) polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies
• Alternative to gene target
  SMAD family member 2, hMAD-2 and hSMAD2 and JV18 and JV18-1 and MADH2 and MADR2, SMAD2 and IDBG-3127 and ENSG00000175387 and 4087, transforming growth factor beta receptor, nuclei, Smad2 and IDBG-159056 and ENSMUSG00000024563 and 17126, SMAD2 and IDBG-644642 and ENSBTAG00000039916 and 515125,516010

Gene info

• Identity
  HGNC:6768
• Gene
  SMAD2
• Long gene name
  SMAD family member 2
• Synonyms gene
  • MADH2
• Synonyms gene name
  • MAD, mothers against decapentaplegic homolog 2 (Drosophila)
  • SMAD, mothers against DPP homolog 2 (Drosophila)
• Synonyms
  • MADR2
  • JV18-1
• GenBank acession
  • U65019
• Locus
  18q21.1
• Discovery year
  1996-11-15
• Entrez gene record
  4087
• Pubmed identfication
  • 8752209
  • 8673135
• RefSeq identity
  • NM_005901
• Classification
  • SMAD family
• VEGA ID
  OTTHUMG00000132652

Gene info

• Identity
  HGNC:6436
• Gene
  KRT19
• Long gene name
  keratin 19
• Synonyms gene name
  • keratin 19, type I
• Synonyms
  • K19
  • CK19
  • K1CS
  • MGC15366
• Synonyms name
  • keratin, type I cytoskeletal 19
  • keratin, type I, 40-kd
  • cytokeratin 19
  • 40-kDa keratin intermediate filament
• Locus
  17q21.2
• Discovery year
  1988-05-11
• Entrez gene record
  3880
• Pubmed identfication
  • 16831889
• RefSeq identity
  • NM_002276
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133422

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products