pCR-BluntII-TOPO-ROBO4 Plasmid

• Catalog number
  PVTB00491
• Price
  380.85 USD
• Size
  2 ug

• Group
  PCR, polymerase chain reaction
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
• Properties
  Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
• Kit
  Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.

• Gene target
  BluntII-TOPO-ROBO4  
• Gene symbol
  ROBO4
• Short name
  pCR-BluntII-TOPO-ROBO4 Plasmid
• Technique
  plasmid, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. plasmids in 1
• Alternative name
  PCR test kit-BluntII-TOPO-ROBO4 Plasmid
• Alternative technique
  plasmids, dna-amplification

Gene info

• Identity
  HGNC:17985
• Gene
  ROBO4
• Long gene name
  roundabout guidance receptor 4
• Synonyms gene name
  • roundabout homolog 4 (Drosophila)
  • roundabout, axon guidance receptor, homolog 4 (Drosophila)
• Synonyms
  • FLJ20798
  • MRB
  • ECSM4
• Synonyms name
  • magic roundabout
• GenBank acession
  • AF361473
• Locus
  11q24.2
• Discovery year
  2002-01-22
• Entrez gene record
  54538
• Pubmed identfication
  • 11076864
  • 16481322
• RefSeq identity
  • NM_019055
• Classification
  • I-set domain containing
  • Fibronectin type III domain containing
• VEGA ID
  OTTHUMG00000165936

MeSH Data

• Name
  Polymerase Chain Reaction
• Concept
  Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
• Tree numbers
  • E05.393.620.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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