CSTB, human recombinant
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Catalog number9235-10
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PricePlease ask
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Size10 µg
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SynonymsCystatin-B, Stefin B, PME, CST6
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Alternative_namesCystatin-B, Stefin B, PME, CST6
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DescriptionAn anti-protease implicated in myoclonus epilepsy.
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RecombinantYes
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SourceE.coli
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Purity by SDS PAGE>95%
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AssaySDS-PAGE
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Purity>95%
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Biological activityIC50 value is < 3nM
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Molecular WeightThis protein is fused with a 6× His tag at N-terminus and has a calculated MW of 13 kDa.
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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Concentration1 mg/ml
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AppearanceLiquid
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Physical form descriptionLiquid
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Reconstitution InstructionsIn 20 mM Tris-HCl buffer (pH8.0) containing 50mM NaCl.
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Background InformationCSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to form a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes. In cells, CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus.
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Amino acid sequenceMGSSHHHHHH SSGLVPRGSH MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYF
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolCSTB
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Short nameCSTB, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative namecystatin B (stefin B), H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targetcystatin B (stefin B), CST6 and EPM1 and EPM1A and PME and STFB and ULD, CSTB and IDBG-4888 and ENSG00000160213 and 1476, poly(A) RNA binding, nuclei, Cstb and IDBG-169100 and ENSMUSG00000005054 and 13014
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Gene info
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Identity
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Gene
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Long gene namecystatin B
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Synonyms gene
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Synonyms gene name
- cystatin B (stefin B)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-12-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cystatins, type 1
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data