GRAP Antibody, ALEXA FLUOR 488
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Catalog number
bs-13640R-A488
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Price
Please ask
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Size
100ul
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Long name
GRAP Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
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Also known as
Anti-GRAP PAb ALEXA FLUOR 488
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Category
Conjugated Primary Antibodies
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Conjugated with
ALEXA FLUOR® 488
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
GRAP
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Specificity
This is a highly specific antibody against GRAP.
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Modification
Unmodified
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Modification Site
None
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human GRAP
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the antigen
Many growth factors function by binding receptors with intrinsic tyrosine kinase activity. Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and specific receptor sequences. For instance, the GRB family of proteins lack a defined catalytic activity and are entirely composed of SH2 and SH3 domains. Members include GRB2, GRB7, GRB10 (also referred to as GRB-IR), GRB14 and Grap (for GRB2-related adapter protein). While GRB10 and GRB14 are most closely related to GRB7, Grap shares the highest degree of homology with GRB2 exhibiting 59% sequence identity with GRB2. The Grap SH2 domain is capable of binding to the activated stem cell factor receptor, c-Kit and the erythropoietin receptor (EpoR). Grap also associates with the Ras guanine nucleotide exchange factor Sos 1 via its amino terminal SH3 domain.
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Purification
Purified by Protein A.
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Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
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Excitation emission
499nm/519nm
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Synonyms
Grap; GRAP_HUMAN; GRB2 related adaptor protein; GRB2-related adapter protein; Growth factor receptor bound protein 2 related adaptor protein; MGC64880; OTTHUMP00000065766.
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Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-GRAP has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
Alexa Fluor
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French translation
anticorps
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Gene target
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Gene symbol
GRAP
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Short name
Anti-GRAP
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Immunoglobulin G (IgG)
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Label
ALEXA FLUOR 488
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Alternative name
GRAP Polyclonal Antibody, ALEXA FLUOR 488
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Alternative technique
antibodies
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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