P53 protein(wt-p53) Antibody, Cy5.5 Conjugated
#
-
Catalog numberbs-0033R-Cy5.5
-
Price:
-
Size0.1ml
-
-
Long nameP53 protein(wt-p53) Polyclonal Antibody, Cy5.5 Conjugated
-
CategoryConjugated Primary Antibodies
-
ConjugationCy5.5
-
Host OrganismRabbit (Oryctolagus cuniculus)
-
Target AntigenP53 proteinwt-p53
-
SpecificityThis is a highly specific antibody against P53 proteinwt-p53.
-
ModificationUnmodified
-
Modification siteNone
-
ClonalityPolyclonal
-
ClonePolyclonal antibody
-
Concentration1ug per 1ul
-
Immunogen_range240-290/393
-
Subcellular locationCytoplasm, Nucleus
-
SourceKLH conjugated synthetic peptide derived from human P53
-
Gene ID number7157
-
Swiss ProtP04637
-
Tested applicationsIF(IHC-P), IF(ICC)
-
Recommended dilutionsIF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
-
CrossreactivityHuman, Mouse, Rat
-
Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Antigen backgroundActs as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
-
Purification methodThis antibody was purified via Protein A.
-
Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
-
Excitation Emission675nm/694nm
-
SynonymsP53; BCC7; LFS1; TRP53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53
-
PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.
-
Additional conjugationCy5.5, Cy5.5
-
ConjugatedThese antibodies are excite for emission at 678nm and detected at a 694 nm wavelengths.
-
French translationanticorps
-
Gene target
-
Gene symbolTP53
-
Short nameAnti-P53 proteinwt-p53
-
TechniqueAntibody, antibodies against human proteins, antibodies for, antibody Conjugates
-
IsotypeImmunoglobulin G (IgG)
-
LabelCy5.5
-
Alternative nameAnti-P53 proteinwt-p53 PAb Cy5.5
-
Alternative techniqueantibodies
-
Gene info
-
Identity
-
Gene
-
Long gene nametumor protein p53
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1986-01-01
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data