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Alternative_name
FITC-Ile-Glu-Thr-Asp-fluoromethyl ketone; FITC-I-E(OMe)-TD(OMe)-FMK
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Description
FITC-IE(OMe)TD(OMe)-FMK is a fluorescein isothiocyanate (FITC) conjugate of the cell- permeable caspase-8 inhibitor IE(OMe)TD(OMe)-FMK that acts as an in situ marker for detection of caspase-8 in living cells. FITC-IE(OMe)TD(OMe)-FMK is nontoxic, and irreversibly binds to activated caspase-8 in apoptotic cells. The bound marker is localized by fluorescence detection.
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Structure Available
Yes
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Peptide sequence
FITC-I-E(OMe)-TD(OMe)-FMK
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Salt Form
No
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Molecular Formula
C₄₃H₄₈FN₅O₁₄S
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Molecular Weight
909.93
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Cell Permeable
Yes
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Purity
≥98% by HPLC
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Handling
Protect from air and moisture
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Tag Line
A caspase-8 in situ marker
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
36 months
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SMILES
S/C(N[C@@H]([C@@H](C)CC)C(N[C@H](C(N[C@@H]([C@H](O)C)C(N[C@H](C(CF)=O)CC(OC)=O)=O)=O)CCC(OC)=O)=O)=N\C1=CC=C(C2(C3=CC=C(O)C=C3OC4=C2C=CC(O)=C4)OC5=O)C5=C1
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InChi
InChI=1S/C43H48FN5O14S/c1-6-20(2)36(39(57)46-29(13-14-34(54)60-4)38(56)48-37(21(3)50)40(58)47-30(31(53)19-44)18-35(55)61-5)49-42(64)45-22-7-10-26-25(15-22)41(59)63-43(26)27-11-8-23(51)16-32(27)62-33-17-24(52)9-12-28(33)43/h7-12,15-17,20-21,29-30,36-37,50-52H,6,13-14,18-19H2,1-5H3,(H,46,57)(H,47,58)(H,48,56)(H2,45,49,64)/t20-,21+,29-,30-,36-,37-/m0/s1
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InChi Key
ZQSUFEPGEWYBEF-NINMPSEZSA-N
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MSDS Available
Yes
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Properties
This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
