Protein A/G-FITC

• Catalog number
  6504-1
• Price
  Please ask
• Size
  1 mg

• Synonyms
  Protein A/G
• Alternative_names
  Protein A/G
• Description
  Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥97%
• Assay
  SDS-PAGE
• Purity
  ≥97%
• Binding Capacity
  Under optimal conditions, 1 mg protein A/G will bind approximately 5 mg human IgG. Optimal binding of Protein A/G to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.
• Molecular Weight
  59.7 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized with no additives
• Reconstitution Instructions
  Reconstitution in H₂O to a concentration of 5 mg/ml gives a clear solution.
• Background Information
  Protein A/G is a genetically engineered protein that combines the IgG binding profiles of both Protein A and Protein G. It is a gene fusion product. Recombinant fusion protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the fusion protein A/G to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G detection using anti-His-tag antibody. Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies. Protein A/G-FITC is prepared by conjugating recombinant Protein A/G to FITC. The conjugation was optimized to provide the maximal binding capacity for IgG and also maximum FITC fluorescence.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  Protein   A/G  
• Gene symbol
  GPRC6A, GPR162, PURG
• Short name
  Protein A/G-FITC
• Technique
  FITC, Fluorescein
• Label
  FITC
• Alternative name
  Protein A/G-fluorecein
• Alternative technique
  fluorescine

Gene info

• Identity
  HGNC:18510
• Gene
  GPRC6A
• Long gene name
  G protein-coupled receptor class C group 6 member A
• Synonyms gene name
  • G protein-coupled receptor, family C, group 6, member A
  • G protein-coupled receptor, class C, group 6, member A
• Synonyms
  • bA86F4.3
• GenBank acession
  • AF502962
• Locus
  6q22.1
• Discovery year
  2002-04-26
• Entrez gene record
  222545
• Classification
  • Calcium sensing receptors
• VEGA ID
  OTTHUMG00000015447

Gene info

• Identity
  HGNC:16693
• Gene
  GPR162
• Long gene name
  G protein-coupled receptor 162
• Synonyms
  • A-2
  • GRCA
• GenBank acession
  • U47928
  • U47929
  • U47924
  • U47925
• Locus
  12p13
• Discovery year
  2005-05-09
• Entrez gene record
  27239
• Pubmed identfication
  • 15777626
• RefSeq identity
  • NM_019858
• Classification
  • G protein-coupled receptors, Class A orphans
• VEGA ID
  OTTHUMG00000168368

Gene info

• Identity
  HGNC:17930
• Gene
  PURG
• Long gene name
  purine rich element binding protein G
• Synonyms
  • PURG-A
  • PURG-B
• GenBank acession
  • AF195513
• Locus
  8p12
• Discovery year
  2004-02-09
• Entrez gene record
  29942
• Pubmed identfication
  • 12034829
• RefSeq identity
  • NM_013357
• VEGA ID
  OTTHUMG00000157357

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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