EZDetect™ Aldo-keto Reductase Activity Assay Kit (Colorimetric)

• Catalog number
  K847-100
• Price
  Please ask
• Size
  100 assays

• Description
  Market's first enzymatic AKR activity assay. Simple and Ultrasensitive.
• Summary
  Simple and Ultrasensitive assay to colorimetrically measure as low as 0.001 mU of AKR activity.
• Detection Method
  Absorbance (450 nm)
• Species Reactivity
  Mammalian
• Applications
  • Measurement of aldo-keto reductase activity in various tissues/cells. • Screening of aldo-keto reductase inhibitors.
• Sample Type
  • Animal tissues: such as liver, pancreas, small intestine, etc. • Cells: Adherent and suspension cells. • Biological fluids: such as serum and plasma.
• Features Benefits
  Simple, ultra-sensitive assay. Colorimetric detection
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Aldo-keto Reductases (AKRs) are a superfamily of NAD(P)H linked oxidoreductases which contains more than 190 members. A complete listing of AKR superfamily can be found at: more The common function of AKR superfamily is to oxidize/reduce aldehydes or ketones to their corresponding primary or secondary alcohols. Due to their broad substrate specificity, AKRs play roles in intermediary metabolism, biosynthesis and detoxification. In humans, the AKRs are not only involved in carbonyl metabolism, but they also regulate signaling through nuclear receptors which leads to chemical carcinogenesis. Many studies demonstrate increasing expression of AKRs in cancers such as lung, liver and colon cancers. BioVision’s EZDetect™ Aldo-keto Reductase Activity Assay Kit provides a convenient tool for sensitive detection of AKR activity in a variety of samples. In this assay, AKR acts on a general substrate and converts NADP+ to NADPH. This NADPH reacts with the AKR probe and generates color. This color is proportional to the activity of AKR in the sample. This kit can detect AKR activity as low as 0.001 mU in samples and has been validated with AKR1B10, AKR1C1 and AKR1C3. Further tests are in progress for other members of the AKR family.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  EZDetect™   Aldo-keto   Reductase   Activity   Kit  
• Short name
  EZDetect™ Aldo-keto Reductase Activity Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  EZDetect™ Aldo-keto Reductase Activity test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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