NLE1 Antibody, ALEXA FLUOR 647

• Catalog number
  bs-12401R-A647
• Price
  Please ask
• Size
  100ul

• Long name
  NLE1 Polyclonal Antibody, ALEXA FLUOR 647 Conjugated
• Also known as
  Anti-NLE1 PAb ALEXA FLUOR 647
• Category
  Conjugated Primary Antibodies
• Conjugated with
  ALEXA FLUOR® 647
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  NLE1
• Specificity
  This is a highly specific antibody against NLE1.
• Modification
  Unmodified
• Modification Site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human NLE1
• Gene ID Number
  54475
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the antigen
  The Notch signaling pathway is an evolutionary conserved system that is involved in intracellular communication. Notch receptors play an important role in development and cell-fate decisions. Notchless is a loss-of-function mutant allele that encodes for protein NLE1 (notchless homolog 1). NLE1 is a 485 amino acid WD40-repeat protein that binds to the cytoplasmic domain of Notch, regulating its signaling activity in Drosophila melanogaster and in mice. Deletion of the NLE1 gene in mice during the early stages of development results in embryonic death, while gene deletion in the late stages of development leads to activation of a caspase-3-dependent apoptotic pathway. In plants, NLE1 is crucial for normal cellular growth and development. Under-expression during shoot proliferation causes pleiotropic defects such as delayed flowering and abnormal organ maturation. It may also play a role in 60S ribosomal subunit biogenesis in yeast. NLE1 contains eight WD40 domains and produces one isoform due to alternative splicing.
• Purification
  Purified by Protein A.
• Storage conditions
  Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
• Excitation emission
  650nm/665nm
• Synonyms
  FLJ10458; NLE 1; Nle; Notchless gene homolog; Notchless homolog 1 Drosophila; Notchless homolog 1; Notchless protein homolog 1; NLE1_HUMAN.
• Properties
  For facs or microscopy Alexa 1 conjugate. Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-NLE1 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  Alexa Fluor, ALEXA FLUOR 647
• French translation
  anticorps

• Gene target
  NLE1  
• Gene symbol
  NLE1
• Short name
  Anti-NLE1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 647
• Alternative name
  NLE1 Polyclonal Antibody, ALEXA FLUOR 647
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:19889
• Gene
  NLE1
• Long gene name
  notchless homolog 1
• Synonyms gene
  • NLE
• Synonyms gene name
  • notchless homolog (Drosophila)
• Synonyms
  • FLJ10458
• Synonyms name
  • Notchless gene homolog, (Drosophila)
• Locus
  17q12
• Discovery year
  2005-05-24
• Entrez gene record
  54475
• RefSeq identity
  • NM_018096
• Classification
  • WD repeat domain containing
• VEGA ID
  OTTHUMG00000132928

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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