UBQ11, Ubiquitin protein (positive control)

• Catalog number
  AS10 307S
• Price
  Please ask
• Size
  50 µl

• Available ordering format
  Liquid
• Immunogen
  to be determined
• Raised in
  N/A
• Clonality
  N/A
• Clone
  N/A
• Purification
  Affinity purified
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  store protein standard at -20°C; Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
• Verified applications
  Western blot (WB)
• Connected products
  AS08 307 | UBQ11 | Ubiquitin antibodies
• Recommended dilutions for use
  positive control: a 2.5-5 μl load per well is optimal for most chemiluminescent detection systems.
• Molecular weight expected аpparent
  10-12 kDa
• Verified reactivity
  to be determined
• Possible reactivity
  to be determined
• No reactivity
  no confirmed exceptions from predicted reactivity currently known
• Supplementary information
  Concentration: 0.8 µg/µl. Recommended load per gel to allow Coomasie staining and western blot detection is 2.5-5 µl.1x SDS Loading Buffer contains:50 mM Tris-HCl pH 6.82% SDS10% glycerol1% β-mercaptoethanol12.5 mM EDTA0.02% bromophenol blue
• References
  to be added when available
• Scientific context
  Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS).Source of positive control, ubiquitin protein: full length recombinant Arabidopsis thaliana ubiquitin, ubq11, with N-terminal 1XHis-3XHA-tag; locus accession: At4g05050
• Notes
  Protein is provided in Laemli SDS loading buffer and should be heated up in 98°C for 5 minutes before loading on the gel.
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples. Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.
• Group
  positif

• Gene target
  UBQ11   Ubiquitin   protein  
• Short name
  UBQ11, Ubiquitin protein (positive control)
• Technique
  Positive, Control, controls
• Alternative name
  UBQ11, Ubiquitin protein (positive reference)
• Alternative technique
  controls
• Tissue
  control

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