CNTF, human recombinant

• Catalog number
  P1093-50
• Price
  Please ask
• Size
  50 µg

• Synonyms
  Ciliary neurotrophic factor, CNTF
• Alternative_names
  Ciliary neurotrophic factor, CNTF
• Description
  Survival factor for various neuronal cell types.
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 1 EU/ug
• Activity Specifications test method
  Measured in a cell proliferation assay using TF-1 human erythroleukemic cell.
• Results
  The ED50 for this effects is less or equal to 1 ug/ml.
• Molecular Weight
  25 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  In 20 mM Tris-HCl buffer (pH 8.5) containing 1 mM DTT, 30% Glycerol, 0.2M NaCl
• Background Information
  Ciliary neurotrophic factor, CNTF
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEAERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLKVLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CNTF  
• Gene symbol
  ZFP91-CNTF, CNTF
• Short name
  CNTF, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  ciliary neurotrophic factor, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  ciliary neurotrophic factor, HCNTF, CNTF and IDBG-409278 and ENSG00000242689 and 1270, growth factor activity, Extracellular, Cntf and IDBG-254935 and ENSMUSG00000079415 and 12803, CNTF and IDBG-637978 and ENSBTAG00000003624 and 100848228

Gene info

• Identity
  HGNC:33441
• Gene
  ZFP91-CNTF
• Long gene name
  ZFP91-CNTF readthrough (NMD candidate)
• GenBank acession
  • AB057443
• Locus
  11q12.1
• Discovery year
  2011-05-31
• Entrez gene record
  386607
• Pubmed identfication
  • 12738986
• RefSeq identity
  • NR_024091
• VEGA ID
  OTTHUMG00000166280

Gene info

• Identity
  HGNC:2169
• Gene
  CNTF
• Long gene name
  ciliary neurotrophic factor
• Synonyms
  • HCNTF
• Synonyms name
  • Ciliary Neuronotrophic Factor
• GenBank acession
  • BC068030
• Locus
  11q12.1
• Discovery year
  1991-01-07
• Entrez gene record
  1270
• Pubmed identfication
  • 1840538
  • 1714745
• RefSeq identity
  • NM_000614
• Classification
  • Interleukin 6 type cytokine family
• VEGA ID
  OTTHUMG00000137476
• Locus Specific Databases
  • ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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