CNTF, human recombinant

• Catalog number
  4017-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  HCNTF, CNTF, Ciliary Neurotrophic Factor.
• Alternative_names
  HCNTF, CNTF, Ciliary Neurotrophic Factor.
• Description
  A potent neural factor promoting survivability and differentiation of neuronal cell types.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Purity
  ≥98%
• Biological activity
  The ED₅₀ as determined by the dose-dependent proliferation of human TF-1 cells is < 2 ng/ml, corresponding to a specific activity of > 5 x 10⁵units/mg.
• Results
  < 2 ng/ml
• Molecular Weight
  22.9 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Sterile filtered and lyophilized without additives
• Reconstitution Instructions
  Reconstitute in 5 mM Sodium Phosphate, pH 7.5 to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use
• Background Information
  CNTF (Ciliary Neurotrophic Factor) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro. CNTF has also been shown to promote survivability and differentiation of other neuronal cell types. Recombinant human CNTF is a 22.9 kDa protein containing 200 amino acid residues
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CNTF  
• Gene symbol
  ZFP91-CNTF, CNTF
• Short name
  CNTF, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  ciliary neurotrophic factor, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  ciliary neurotrophic factor, HCNTF, CNTF and IDBG-409278 and ENSG00000242689 and 1270, growth factor activity, Extracellular, Cntf and IDBG-254935 and ENSMUSG00000079415 and 12803, CNTF and IDBG-637978 and ENSBTAG00000003624 and 100848228

Gene info

• Identity
  HGNC:33441
• Gene
  ZFP91-CNTF
• Long gene name
  ZFP91-CNTF readthrough (NMD candidate)
• GenBank acession
  • AB057443
• Locus
  11q12.1
• Discovery year
  2011-05-31
• Entrez gene record
  386607
• Pubmed identfication
  • 12738986
• RefSeq identity
  • NR_024091
• VEGA ID
  OTTHUMG00000166280

Gene info

• Identity
  HGNC:2169
• Gene
  CNTF
• Long gene name
  ciliary neurotrophic factor
• Synonyms
  • HCNTF
• Synonyms name
  • Ciliary Neuronotrophic Factor
• GenBank acession
  • BC068030
• Locus
  11q12.1
• Discovery year
  1991-01-07
• Entrez gene record
  1270
• Pubmed identfication
  • 1840538
  • 1714745
• RefSeq identity
  • NM_000614
• Classification
  • Interleukin 6 type cytokine family
• VEGA ID
  OTTHUMG00000137476
• Locus Specific Databases
  • ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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