Rabbit Anti-Human PAK1 (pSer144) Polyclonal: APC
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Catalog numberSPC-1043D-APC
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Price:
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Size100 µl
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ImmunogenA phospho-specific peptide corresponding to residues surrounding Ser144 of human PAK1 (AA141-147)
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Antibody s targetHuman PAK1 (pSer144)
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Antibody s full descriptionRabbit Anti-Human PAK1 (pSer144) Polyclonal
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameADRB2 Antibody, Alpha PAK Antibody, Alpha-PAK Antibody, p21 activated kinase 1 Antibody, p21 protein (Cdc42/Rac) activated kinase 1 Antibody, p21-activated kinase 1 Antibody, p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) Antibody, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) Antibody, p65 PAK Antibody, p65-PAK Antibody, P68-PAK Antibody, PAK alpha Antibody, PAK-1 Antibody, Pak1 Antibody, PAK1_HUMAN Antibody, Paka Antibody, PAKalpha Antibody, Protein kinase MUK2 Antibody, Rac/p21-activated kinase Antibody, Serine/threonine-protein kinase PAK 1 Antibody, STE20 homolog yeast Antibody
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Verified applicationsWB, AM
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Raised inRabbit
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Antibody s reactivityHuman
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Antibody s recommended dilutions for useWB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects 60.661 kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:250 dilution of SPC-1043 was sufficient for detection of PAK1 (pSer144) in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm, Cell Junction, Focal Adhesion, Cell Membrane, Cell Projection, Invadopodium
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Tissue specificityHighly expressed in the brain and blood cells. Overexpressed in gastric cancer cells and tissues.
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Scientific contextPAK1 is a protein-serine/threonine kinase. Pak1 binds to activated forms of the GTPases Cdc42 and Rac. Upon binding to Cdc42 or Rac, Pak1 is activated and autophosphorylates. Pak1 has dozens of substrates, activating cytoskeletal and transcription pathways that enhance cell motility, proliferation, and survival.
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BibliographyRefer to PubMed
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Released date29/Aug/2016
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NCBI numberNP_002567
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Gene number5058
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Protein numberQ13153
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure linkNo Data Available
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolPAK1, APC, PKN1
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Short nameRabbit Anti- PAK1 (pSer144) Polyclonal: APC
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelAPC
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman p21 protein (Cdc42/Rac)-activated kinase 1 (pSer144) polyclonal: adenomatous polyposis coli
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Alternative to gene targetadenomatous polyposis coli, BTPS2 and DP2 and DP2.5 and DP3 and GS and PPP1R46, APC and IDBG-37007 and ENSG00000134982 and 324, microtubule plus-end binding, nuclei, Apc and IDBG-134573 and ENSMUSG00000005871 and 11789, BT.25770 and IDBG-644937 and ENSBTAG00000018852 and 533233
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Gene info
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Identity
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Gene
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Long gene namep21 (RAC1) activated kinase 1
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Synonyms gene name
- p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
- p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
- p21 protein (Cdc42/Rac)-activated kinase 1
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-12-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameAPC regulator of WNT signaling pathway
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Synonyms gene name
- adenomatosis polyposis coli
- adenomatous polyposis coli
- APC, WNT signaling pathway regulator
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein phosphatase 1 regulatory subunits
- Armadillo repeat containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameprotein kinase N1
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Synonyms gene
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Synonyms gene name
- protein kinase C-like 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-10-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C2 domain containing protein kinases
- AGC family kinases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data