HDAC1 Immunoprecipitation (IP) & Activity Assay Kit
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Catalog number
K342-25
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Price
Please ask
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Size
25 assays
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Description
Only kit on the market for both IP and measuring activity of HDAC1
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Summary
• Detection method - Fluorescence (Ex/Em=380/500 nm) • Applications: - Immunoprecipitation of HDAC1 complex from cell, nuclear, or tissue extract(s) - Measurement of HDAC1 activity of immunoprecipitated complex/purified enzyme - Screening for activators or inhibitors of HDAC1
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Sample Type
Human, Mouse or Rat • Cell Lysate and Nuclear Extract • Tissue Extract
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Features Benefits
• Simple, fast and convenient. • Only kit on the market for both IP as well as measuring activity of HDAC1
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 moths
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Background
Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. HDAC1 is ubiquitously expressed and plays a role in cellular senescence, cardiomyocyte differentiation and hypertrophy, aging of the liver, myelination, and adult neurogenesis to name a few functions. The HDAC1 IP & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC1 complex from cells & tissues and to measure HDAC1 activity fluorometrically. HDAC1 is immunoprecipitated from cell, nuclear or tissue extract(s) using HDAC1 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
HDAC1
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Short name
HDAC1 Immunoprecipitation (IP) & Activity Assay Kit
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Technique
Immunoprecipitation, Assay, IP, assays, Immunoprecipitation
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Host
Assay
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Alternative name
histone deacetylase 1 Immunoprecipitation (IP) & Activity test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
histone deacetylase 1
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year
1996-11-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- NuRD complex subunits
- Histone deacetylases, class I
- MiDAC complex subunits
- EMSY complex
- SIN3 histone deacetylase complex subunits
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VEGA ID
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