LDHA Antibody (Center)

• Catalog number
  6736-100
• Price
  Please ask
• Size
  100 μl

• Antibody Target
  LDHA
• Target Alternative Name
  LDHA; L-lactate dehydrogenase A chain; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; Renal carcinoma antigen NY-REN-59.
• Antibody Type
  Polyclonal
• Species Reactivities
  Human. Predicted cross reactivity with monkey, pig, rat and bovine samples.
• Immunogen Sequence
  This LDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-182 amino acids from the Central region of human LDHA.
• Accession
  P00338
• Gene Id
  3939
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  100 µl of antibody in PBS with 0.09% (W/V) sodium azide
• Purification
  This antibody is purified through a protein A column, followed by peptide affinity purification.
• Application
  Western blot, IHC, FACS.
• Positive Control
  Western blot: Ramos and A375 cell line lysates, IHC: skin tissue, FACS: HeLa cells
• Application And Usages
  Western blot: ~1:1000, IHC: ~1:50–1:100, FACS: ~1:10-1:50.
• Description
  L-Lactate dehydrogenase A chain (LDHA) is a member of the LDH/MDH superfamily and LDH family. It catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA is localized primarily in muscle tissue and is part of the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. LDH1 is decreased in essential thrombocythemia. LDHA is induced through a non-genomic pathway of estrogen action. Reduction in LDH-A activity results in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. Mutations in LDHA have been associated with in LDHA are the cause of glycogen storage disease type 11 (GSD11) which is a metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  LDHA   Center  
• Gene symbol
  LDHA
• Short name
  LDHA Antibody (Center)
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  LDHA (antibody to-) (central)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6535
• Gene
  LDHA
• Long gene name
  lactate dehydrogenase A
• GenBank acession
  • X02152
• Locus
  11p15.1
• Discovery year
  2001-06-22
• Entrez gene record
  3939
• Pubmed identfication
  • 3000353
• RefSeq identity
  • NM_005566
• VEGA ID
  OTTHUMG00000167721

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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