IgG1 antibody conjugated to FITC - IgG1 antibody conjugated to PE - Negative Control
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Catalog number
G1G1
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Price
Please ask
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Size
100 Tests
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Category
Negative Control
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Long description
Negative control for Immunofluorescence staining with FITC and PE of mouse monoclonal antibodies. Assessment of non-specific binding of mouse monoclonal antibodies to human cell surface antigens.
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Antibody come from
n/a
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Other description
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
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Clone
not specified
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Antigen antibody binding interaction
Mouse IgG1 antibody conjugated to FITC - IgG1 antibody conjugated to PE - Negative Control
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Antibody is raised in
Mouse
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Antibody s reacts with
see techfile
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Flow Cytometry
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Antibody s suited for
Consult the appropriate fact sheet to determine the amount of antibodies to be used as a control for PBMC or Whole Blood. PBMC: Generally, add 10 µl of MAB/10^6 PBMC in100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20¼ C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
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Storage
4ºC
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Short name
IgG1 antibody conjugated FITC - IgG1 antibody conjugated PE - Negative Control
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Technique
Antibody, Negative, Control, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, controls, Fluorescein, negatif
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Isotype
IgG1, IgG1
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Label
FITC and PE
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Alternative name
IgG1 (antibody to-) coupled to fluorecein - IgG1 (antibody to-) coupled to peroxidase - Negative reference
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Alternative technique
controls, antibodies, fluorescine
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Tissue
control
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MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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