Glyoxalase II Activity Kit (Colorimetric)

• Catalog number
  K460-100
• Price
  Please ask
• Size
  100 assays

• Description
  The most sensitive kit in the market to detect Glyoxalase II activity
• Summary
  • Detection method- Absorbance (450 nm) • Sample type- tissue and cell culture lysate, red blood cells • Species reactivity- Mammalian • Application- Measurement of Glyoxalase II as low as 0.6 mU/ml.
• Detection Method
  Colorimetric (OD 450 nm)
• Species Reactivity
  Mammalian
• Applications
  Measurmente of Glyoxalase II activity in biological samples
• Sample Type
  Cell and tissue lysates, red blood cells
• Features Benefits
  • Simple procedure; takes ~ 40 minutes • Fast and convenient • Kit contains all necessary reagents for accurate measurement of Glyoxalase II
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Glyoxalase II (GloII, hydroxyacylglutathione hydrolase, EC 3.1.2.6) is one of the two enzymes in the Glyoxalase system that is ubiquitously expressed in mammalian, plants and bacteria. The Glyoxalase system is a biological pathway that detoxifies cells by metabolizing α-ketoaldehydes such as methylglyoxal, a cytotoxic byproduct of lipids and glucose metabolism. GloII fulfills the terminal step in the glyoxalase system: It catalyzes the hydrolysis of S-D-lactoyl-glutathione (SLG) to reduced glutathione and D-lactic acid. The alteration of GloII activity in cell growth cycle, cell differentiation, phagocyte activation and diabetes mellitus development of implies this enzyme plays a wide-ranging and important role in cell function and disease progression. Research areas of current interest in Glo-II include diabetes, and cancer therapy. Biovision’s Glyoxalase II Activity Kit utilizes the ability of an active Glo-II to cleave a substrate while producing D-lactate. The produced D-Lactate reacts with the chromophore generating a stable signal that can be easily quantified at 450 nm using a microplate reader. Our assay kit is simple, sensitive and can detect as low as 0.6 mU/ml of Glo-II activity in biological samples
• Data Sheet Link
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Glyoxalase   Activity   Kit  
• Short name
  Glyoxalase II Activity Kit (Colorimetric)
• Technique
  colorimetric, colorimetry
• Alternative name
  Glyoxalase II Activity reagent (colorimetry)
• Alternative technique
  assays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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