USP14 Antibody

• Catalog number
  6134-50
• Price
  Please ask
• Size
  50 μg

• Antibody Target
  USP14
• Target Alternative Name
  Deubiquitinating enzyme 14, TGT, Ubiquitin carboxyl-terminal hydrolase 14, Ubiquitin-specific processing protease 14, Ubiquitin thioesterase 14, Ubiquitin-specific-processing protease 14
• Antibody Type
  Polyclonal
• Species Reactivities
  Human
• Immunogen Sequence
  Recombinant full length protein
• Accession
  P54578
• Gene Id
  9097
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  50 µg of antibody in PBS containing 10% glycerol
• Purification
  Purified from egg yolk
• Application
  Western blot
• Application And Usages
  Western blot: Robust detection of 100 ng of recombinant protein was possible when antibody was used at a final concentration of 5 μg/mL
• Description
  Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. In humans, there are three proteasomal DUBs: PSMD14 (POH1/RPN11), UCH37 (UCH-L5), and Ubiquitin-Specific Protease 14, which is also known as the 60 kDa subunit of tRNA-guanine transglycosylase (USP14/TGT60 kDa). USP14 is recruited to the proteasome through its reversible association with the PSMD2 (S2/hRPN1) subunit of the 19S regulatory particle. Whereas PSMD14 appears to promote substrate degradation, USP14 is thought to antagonize substrate degradation. While the underlying mechanism for the opposing roles of these two proteasomal DUBs is still uncertain, it is thought that USP14 removes ubiquitin from substrate upon docking of the substrate with the 26S proteasome. Furthermore, USP14 trims ubiquitin residues from the distal end of the polyubiquitin chain, thus decreasing the affinity of the chain for the ubiquitin receptors of the proteasome, and allowing for enhanced substrate stability. Studies have elucidated a physiologic role for USP14 in regulating synaptic activity in mammals. Research studies have shown that targeting this activity with small molecule inhibitors has potential benefits for the treatment of neurodegenerative diseases and cancer.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  USP14  
• Gene symbol
  USP14
• Short name
  USP14 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Chicken IgG
• Alternative name
  ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) (antibody to-)
• Alternative technique
  antibodies
• Alternative to gene target
  ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase), TGT, USP14 and IDBG-259 and ENSG00000101557 and 9097, proteasome binding, Cell surfaces, Usp14 and IDBG-128322 and ENSMUSG00000047879 and 59025, BT.20599 and IDBG-644085 and ENSBTAG00000019214 and 505106

Gene info

• Identity
  HGNC:12612
• Gene
  USP14
• Long gene name
  ubiquitin specific peptidase 14
• Synonyms gene name
  • ubiquitin specific protease 14 (tRNA-guanine transglycosylase)
  • ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
• Synonyms
  • TGT
• GenBank acession
  • U30888
• Locus
  18p11.32
• Discovery year
  1999-02-01
• Entrez gene record
  9097
• Pubmed identfication
  • 12838346
• RefSeq identity
  • NM_005151
• Classification
  • Ubiquitin specific peptidases
• VEGA ID
  OTTHUMG00000178040

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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