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Antibody Target
ITGA4
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Target Alternative Name
269C wild type; Antigen CD49D, alpha 4 subunit of VLA 4 receptor; CD49 antigen like family member D; CD49 antigen-like family member D; CD49d; IA4; Integrin alpha 4; Integrin alpha 4 subunit; Integrin alpha IV; Integrin alpha-4; Integrin alpha-IV; Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor); ITA4_HUMAN; ITGA4; mgC90518; OTTHUMP00000205320; Very late activation protein 4 receptor, alpha 4 subunit; VLA 4 subunit alpha; VLA-4 subunit alpha; VLA4
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Antibody Type
Polyclonal
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Species Reactivities
Human, Mouse, Rat
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Immunogen Sequence
A synthesized peptide derived from human ITGA4.
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Accession
P13612
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Gene Id
3676
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Appearance
Colorless liquid
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Form
Liquid
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Concentration
1 mg/ml
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Formulation
In phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol
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Purification
Affinity purified
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Application
WB, IHC, ELISA
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Positive Control
WB: Jurkat cells, IHC: human pancreas tissue
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Application And Usages
WB 1:500-1:2000, IHC 1:50-1:200, ELISA (peptide) 1:20000-1:40000
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Description
Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival. The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities. Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling). A pair of important α4 integrins, α4β1 and α4β7, interact with VCAM-1, fibronectin, and MAdCAM-1 at cell adhesions. Gene knockout and antibody blocking research reveal that α4 integrins play important roles in embryonic liver and heart development and in fetal lymphocyte homing. Phosphorylation at Ser988 within the cytoplasmic tail of integrin α4 blocks binding to paxillin and promotes leading edge migration.
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
ITGA4
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Short name
Anti-ITGA4 Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
Antibody toITGA4 (antibody to-)
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Alternative technique
antibodies
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Alternative to gene target
integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor), CD49D and IA4, ITGA4 and IDBG-76684 and ENSG00000115232 and 3676, cell adhesion molecule binding, Cell surfaces, Itga4 and IDBG-183203 and ENSMUSG00000027009 and 16401, ITGA4 and IDBG-639925 and ENSBTAG00000009256 and 282882
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