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Description
Plasmid miniPREP Kit is used for isolating the plasmid DNA from the cultured bacterial cells.
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Storage
Store at room temperature
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Shelflife
Use before expiry date given on the label
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Sample
NA
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Format
NA
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Operation time
NA
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Elution volume
NA
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Yield
NA
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Endotoxin value
NA
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Contents
Buffer S1 ; Buffer S2 ; Buffer S3; Buffer W1 ; Buffer W2(Add ethanol) ; Buffer E ; RNase A (50mg/mL) PM column ; Collection Tubes
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PHYSICAL AND CHEMICAL PROPERTIES
Buffer S1 Form liquid immiscible in water pH7.6 // Buffer S2 Form liquid immiscible in water pH 13 // Buffer S3 Form liquid immiscible in water pH 4.5 // Buffer W1 Form liquid immiscible in water pH 7.0 // Buffer W2 Form liquid immiscible in water pH 7.5 // Buffer E Form liquid immiscible in water pH 8.5 // RNase A Form liquid immiscible in water pH 6.8
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Uses
For laboratory use
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Ordering
To order Plasmid miniPREP Kit, please use the Cat. Nr.PDP01-0100 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Plasmid miniPREP Kit
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Kit
Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
