Hsp70 Antibody
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Catalog number3097-30T
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Price:
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Size30 µg
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Antibody TargetHsp70
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Target Alternative NameHSPA4, APG2, APG-2, HSP70RY, HS24/P52, mgC131852 , hsp70RY
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat, monkey, hamster, rabbit, bovine
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Immunogen SequenceSynthetic peptide surrounding amino acid 559 of human Hsp70
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AccessionP5462
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Gene Id3306
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation30 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 1% BSA, 0.02% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot
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Application And UsagesWestern blot analysis (1-2 µg/ml). However, the optimal conditions should be determined individually. The antibody is specific for the inducible form of Hsp70 (Hsp72) and does not cross-react with the constitutive Hsc70 (Hsc73).
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DescriptionHsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but are localized within different intracellular compartments. These include: a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus; b) the highly stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus; c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell. It should be noted, however, that there is a constitutive level of Hsp70 expression in some cell types such as primates, e.g., human and monkey. Nevertheless increased expression of Hsp70 is observed in such cells following stress.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolHSPA14, ST13P1, ST13P13
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Short nameHsp70 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameHsp70 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 14
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Synonyms gene name
- heat shock 70kDa protein 14
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-04-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Heat shock 70kDa proteins
Gene info
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Identity
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Gene
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Long gene nameST13, Hsp70 interacting protein pseudogene 1
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Synonyms gene name
- suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein) pseudogene 1
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Locus
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Discovery year2010-08-11
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameST13, Hsp70 interacting protein pseudogene 13
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Synonyms gene name
- suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein) pseudogene 13
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Locus
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Discovery year2010-08-11
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Entrez gene record
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data