PRPS1, human recombinant

• Catalog number
  7828-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Ribose-phosphate pyrophosphokinase 1, ARTS, CMTX5, PPRibP, PRSI, DFN2, DFNX1
• Alternative_names
  Ribose-phosphate pyrophosphokinase 1, ARTS, CMTX5, PPRibP, PRSI, DFN2, DFNX1
• Description
  An enzyme that catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity: > 1 Units/ml. One unit will catalyze the formation of 1.0 µmole of AMP from ATP and ribose-5-phosphate per minute at pH 7.6 at 37ºC.
• Results
  Specific activity: > 1 Units/ml
• Unit Definition
  One unit will catalyze the formation of 1.0 µmole of AMP from ATP and ribose-5-phosphate per minute at pH 7.6 at 37ºC.
• Molecular Weight
  36.9 kDa (338 aa, 1-318 aa + His Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1 mM DTT and 0.1 M NaCl
• Background Information
  PRPS1, also known as ribose-phosphate pyrophosphokinase 1, is ubiquitously expressed in human tissues. This enzyme catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Recombinant human PRPS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MPNIKIFSGS SHQDLSQKIA DRLGLELGKV VTKKFSNQET CVEIGESVRG EDVYIVQSGC GEINDNLMEL LIMINACKIA SASRVTAVIP CFPYARQDKK DKSRAPISAK LVANMLSVAG ADHIITMDLH ASQIQGFFDI PVDNLYAEPA VLKWIRENIS EWRNCTIVSP DAGGAKRVTS IADRLNVDFA LIHKERKKAN EVDRMVLVGD VKDRVAILVD DMADTCGTIC HAADKLLSAG ATRVYAILTH GIFSGPAISR INNACFEAVV VTNTIPQEDK MKHCSKIQVI DISMILAEAI RRTHNGESVS YLFSHVPL
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PRPS1  
• Gene symbol
  PRPS1
• Short name
  PRPS1, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  PRPS1, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:9462
• Gene
  PRPS1
• Long gene name
  phosphoribosyl pyrophosphate synthetase 1
• Synonyms gene
  • DFN2
• Synonyms gene name
  • deafness, X-linked 2, perceptive, congenital
• Synonyms
  • CMTX5
  • DFNX1
• Synonyms name
  • PRS I
  • ribose-phosphate diphosphokinase 1
• GenBank acession
  • X15331
• Locus
  Xq22.3
• Discovery year
  2001-06-22
• Entrez gene record
  5631
• Pubmed identfication
  • 1962753
  • 20021999
• VEGA ID
  OTTHUMG00000022167
• Locus Specific Databases
  • Global Variome shared LOVD
  • LRG_264

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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