-
Synonyms
BenzAltBinuclease, BenzAlt™ Binuclease, Binuclease, Benzonase alternative, endonuclease, Serratia marcescens binuclease, binuclease, benaltbinuclease, unrestricted endonuclease
-
Alternative_names
Serratia marcescens binuclease; unrestricted endonuclea
-
Description
A genetically engineered non-specific endonuclease from Serratia marcescens gene that can digests all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled DNA and RNA.
-
Recombinant
Produced and purified from eukaryotic yeast cells
-
Source
Serratia marcescens gene
-
Purity by SDS PAGE
≥80%
-
Assay
SDS PAGE
-
Endotoxin Level
None
-
Activity Specifications test method
≥ 1.0 x 106 KU/mg
-
Unit Definition
One unit (U) of Binuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min under the condition above, which means complete digestion of 37 ug DNA
-
Molecular Weight
27.9 kDa
-
Storage Temp
4°C
-
Shipping
Gel Pack
-
Shelf Life
24 months
-
Appearance
Liquid
-
Physical form description
Liquid: clear colorless buffered aqueous glycerol solution
-
Reconstitution Instructions
20 mM Tris-Cl (pH 8.0), 2 mM MgCl2, 2 mM NaCl, 50% Glycerol
-
Background Information
BenzAlt™ Binuclease is a genetically engineered non-specific endonuclease from Serratia marcescens gene. It is a perfect alternative for Benzonase (BenzAlt™ ) and is very cost effective. Functionally, this promiscuous nuclease digests all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled DNA and RNA. The enzyme cleaves the phosphodiester bond of nucleic acids, producing 5’ monophosphate terminated oligonucleotides 2-5 bases in length. BenzAlt™ Binuclease is not a sequence dependent nuclease, capable of cleaving the phosphodiester bond at all positions among a nucleic acid chain. The enzyme is produced and purified from eukaryotic yeast cells, without contamination of endotoxin from prokaryotic cells and is highly stable even under harsh industrial conditions. It has no detectable proteinase or esterase activity. BenzAlt™ Binuclease is an ideal tool enzyme in protein purification and DNA/RNA contamination from purified proteins.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
