ARFGEF1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)

DNA lentivector for transduction information

Lentiveral packaging plasmid DNA for non-viral plasmid transfection and direct use in plasmid expression. This DNA can alos be used for packaging into Lentiviral particles for high efficiency transduction and stably integrated expressions. GENTAUR suggests to use our ABM packaging mix LV003 of second generation virusses or the LV053, our 3rd Generation Packaging mixture. pLenti lentiviral plasmids DNAs are stored in 10milliMolar Tris/HCI with 1mM EDTA at a pH of 8 at -25 C. Vectors with selection markers like kanamycin, puromycin or cumate are available.
Lentiviral technical files
Lentivirus references
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Gene target

ARFGEF1 Lentiviral Vector CMV pLenti-GIII-CMV-C-term-HA
Gene symbol

MYO1G, KRT31, KRT32, KRT33B, KRT35, KRT34, ARHGAP45, KRT33A, AKAP13, ARFGEF1-DT
Short name

ARFGEF1 Lentiviral Vector ( ) (CMV) (pLenti-GIII-CMV-C-term-HA)
Technique

Vectors
Species

Human, Humans
Alternative name

ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) Lentiviral integrating Desoxyribonucleic acid sequence (H. sapiens) (cytomegalovirus) (pLenti-GIII-cytomegalovirus-c terminus-HA)
Alternative to gene target

ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited), ARFGEP1 and BIG1 and P200, ARFGEF1 and IDBG-24344 and ENSG00000066777 and 10565, protein kinase A regulatory subunit binding, nuclei, Arfgef1 and IDBG-133303 and ENSMUSG00000067851 and 211673, ARFGEF1 and IDBG-644666 and ENSBTAG00000014691 and 281022
Virus

cmv

Identity

HGNC:13880
Gene

MYO1G
Long gene name

myosin IG
Synonyms
- HA-2
Synonyms name
- minor histocompatibility antigen HA-2
GenBank acession
- AF380932
Locus

7p13
Discovery year

2000-11-28
Entrez gene record

64005
Classification
- Myosin heavy chains, class I
- Minor histocompatibility antigens
VEGA ID

OTTHUMG00000155821

Identity

HGNC:17102
Gene

ARHGAP45
Long gene name

Rho GTPase activating protein 45
Synonyms gene
- HMHA1
Synonyms gene name
- histocompatibility (minor) HA-1
Synonyms
- KIAA0223
- HA-1
GenBank acession
- D86976
Locus

19p13.3
Discovery year

2005-12-06
Entrez gene record

23526
Pubmed identfication
- 9820596
- 9039502
Classification
- Minor histocompatibility antigens
- AH/BAR family Rho GTPase activating proteins
- F-BAR domain containing

Identity

HGNC:371
Gene

AKAP13
Long gene name

A-kinase anchoring protein 13
Synonyms gene
- LBC
Synonyms gene name
- lymphoid blast crisis oncogene
- A kinase (PRKA) anchor protein 13
Synonyms
- Ht31
- BRX
- AKAP-Lbc
- c-lbc
- PROTO-LB
- HA-3
- ARHGEF13
GenBank acession
- M90360
Locus

15q25.3
Discovery year

1999-09-16
Entrez gene record

11214
Pubmed identfication
- 9627117
- 1860836
RefSeq identity
- NM_007200
Classification
- A-kinase anchoring proteins
- Dbl family Rho GEFs
- Minor histocompatibility antigens
- Pleckstrin homology domain containing
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000172205

Name

Virus Cultivation
Concept

Scope note: Process of growing viruses in live animals, plants, or cultured cells.
Tree numbers
- E01.370.225.875.970
- E05.200.875.970
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data